Effects of Daidzein on mRNA Expression of Bone Morphogenetic Protein Receptor Type I and II Genes in the Ovine Granulosa Cells

  • Chen, A Qin (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Fisheries University) ;
  • Xu, Zi Rong (College of Animal Science of Zhejiang University) ;
  • Yu, Song Dong (College of Animal Science of Zhejiang University) ;
  • Yang, Zhi Gang (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Fisheries University)
  • Received : 2008.05.12
  • Accepted : 2008.11.22
  • Published : 2010.03.01


Daidzein, a natural isoflavonoid phytoestrogen, structurally resembles estradiol (E2) and possesses estrogenic activity. This study was designed to test the hypothesis that daidzein may mimic the effects of E2 on ovine follicle development by regulation of the mRNA expression of bone morphogenetic protein receptor genes and thereby influence the reproductive system. Granulosa cells were cultured in serum-free McCoy's 5A medium with and without supplementation of daidzein. Results showed that daidzein (10-100 ng/ml) significantly increased the proliferation of ovine granulosa cells (p<0.05), but inhibited the growth of granulosa cells at a dose of 1,000 ng/ml (p<0.01). Daidzein inhibited progesterone production in a dose dependent manner; however, it did not affect estradiol production by granulosa cells. We also investigated the effects of daidzein on BMPRII, BMPRIB and ALK-5 mRNA expression in ovine granulosa cells by quantitative real-time PCR. Treatment of granulosa cells with daidzein increased significantly expression of these genes at 10-100 ng/ml. Thus, these data suggested that a low concentration of daidzein (10-100 ng/ml) had a direct stimulatory effect on ovine granulosa cells while a high concentration was toxic.


Daidzein;Granulosa Cells;Cell Proliferation;BMP Receptor Genes


Supported by : Zhejiang Science and Technology Committee


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