Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits

  • Gao, H. ;
  • Fan, B. ;
  • Zhu, M.J. ;
  • Liu, Bang
  • Received : 2006.01.12
  • Accepted : 2009.05.18
  • Published : 2010.01.01


The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).


Full-length cDNA;RH Mapping;Significant Association;Backfat Thickness;PECI Gene;Pigs


  1. Bidanel, J. P. and M. F. Rothschild. 2002. Current status of quantitative trait locus mapping in pigs. Pig News Info. 23(2):39-54
  2. Geisbrecht, Brian V., Dai Zhu, Kerstin Schulz, Katja Nau, James C. Morrell, Michael Geraghtyi, Horst Schulz, Ralf Erdmann and Stephen J. Gould. 1998. Molecular Characterization of Saccharomyces cerevisiae D3,D2-Enoyl-CoA Isomerase. J. Biol. Chem. 273(50):33184-33191
  3. Milan, D., J. P. Bidanel, N. Iannuccelli, J. Riquet, Y. Amigues, J. C. Caritez, J. Gruand, P. Le Roy, H. Lagant, C. Renard and C. Chevalet. 2002. Detection of quantitative trait loci for carcass composition traits in pigs. Genet. Sel. Evol. 34(6):705-728
  4. Palosaari, Päivi M., Johanna M. Kilponen, Raija T. Sormunen, Ilmo E. Hassinen and J. Kalervo Hiltunen. 1990b. $\Delta_{3}$,$\Delta_{2}$- Enoyl-CoA isomerases (Characterization of the mitochondrial isoenzyme in the rat). J. Biol. Chem. 265(6):3347-3353
  5. Yerle, M., P. Pinton and A. Robic. 1998. Construction of a wholegenome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet. Cell Genet. 82(3-4):182-188
  6. Yerle, M., G. Echard, A. Robic, A. Mairal, C. Dubut-Fontana, J. Riquet, P. Pinton, D. Milan, Y. Lahbib-Mansais and J. Gellin. 1996. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell Genet. 73(3):194-202
  7. Janssen, Uwe and Wilhelm Stoffe. 2002. Disruption of mitochondrial $\beta$-oxidation of unsaturated fatty acids in the 3,2-trans-Enoyl-CoA isomerase-deficient mouse. J. Biol. Chem. 277(22):19597-19584
  8. Quintanilla, R., O. Demeure, J. P. Bidanel, D. Milan, N. Iannuccelli, Y. Amigues, J. Gruand, C. Renard, C. Chevalet and M. Bonneau. 2003. Detection of quantitative trait loci for at androstenone levels in pigs. J. Anim. Sci. 81(2):385-394
  9. Nagamine, Y., C. S. Haley, A. Sewalem and P. M. Visscher. 2003. Quantitative trait loci variation for growth and obesity between and within lines of pigs (Sus scrofa). Genetics 164(2):629-635
  10. Palosaari, Paivi M., Mauno Vihinen, Pekka I. Mantsala, Stefan E. H. Alexson, Taina Pihlajaniemi and J. Kalervo Hiltunen. 1991. Amino acid sequence similarities of the mitochondrial short chain $\Delta^{3}$,$\Delta^{2}$-Enoyl-CoA isomerase and peroxisomal multifunctional $\Delta^{3}$,$\Delta^{2}$-Enoyl-CoA isomerase, 2-Enoyl-CoA hydratase, 3-Hydroxyacyl-CoA dehydrogenase enzyme in rat liver. J. Biol. Chem. 266(17):10750-10753
  11. Bidanel, J. P., A. Prunier, N. Iannuccelli and D. Milan. 2001b. Detection of quantitative trait loci for male and female reproductive traits in Meishan×Large White F2 pigs. Page 54 in Proc. 52nd Annual Meeting of the E.A.A.P. Budapest, Hungary
  12. Wang, L., T.-P. Yu, C. K. Tuggle, H.-C. Liu and M. F. Rothschild. 1998. A directed search for quantitative trait loci on chromosomes 4 and 7 in pigs. J. Anim. Sci. 76(10):2560-2567
  13. Chevalet, C., J. Gouzy and M. San Cristobal-Gaudy. 1997. Regional assignment of genetic markers using a somatic cell hybrid panel: a WWW interactive program available for the pig genome. Computer Application Bioscience 13(1):69-73
  14. Partanen, Sanna. T., Dmitry K. Novikov, Alexander N. Popov Anu M. Mursula, J. Kalervo Hiltunen and Rik K. Wierenga. 2004. The 1.3 $\AA$ crystal structure of human mitochondrial $\Delta^{3}$-$\Delta^{2}$- Enoyl-CoA isomerase shows a novel mode of binding for the fatty acyl group. J. Mol. Biol. 342(4):1197-1208
  15. Palosaari, Päivi. M. and J. Kalervo Hiltunen. 1990a. Peroxisomal bifunctional protein from rat liver is a trifunctional enzyme possessing 2-Enoyl-CoA hydratase, 3-Hydroxyacyl-CoA dehydrogenase, and $\Delta_{3}$,$\Delta_{2}$-Enoyl-CoA isomerase activities. J. Biol. Chem. 265(5):2446-2449
  16. Geisbrecht, Brian V., Dongyan Zhang, Horst Schulz, and Stephen J. Gould. 1999. Characterization of PECI, a novel monofunctional Delta (3), Delta(2)-enoyl-CoA isomerase of mammalian peroxisomes. J. Biol. Chem. 274(31):21797-21803
  17. Gurvitz, Aner, Anu M.Mursula, Andreas Firzinger, Barbara Hamilton, Seppo H. Kilpeläinen, Andreas Hartig, Helmut Ruis, J. Kalervo Hiltunnen and Hanspeter Rottensteiner. 1998. Peroxisomal ${\Delta}^{3}$-cis-${\Delta}^{2}$-trans-Enoyl-CoA isomerase encoded by ECI1 is required for growth of the yeast Saccharomyces cerevisiae on unsaturated fatty acids. J. Biol. Chem. 273(47):31366-31374
  18. Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, L. Schook, M. Yerle and J. Gellin. 2000. IMpRH server: an RH mapping server available on the web. Bioinformatics 16(6):558-559
  19. Zhang, Dongyan, Wenfeng Yu, Brian V. Geisbrecht, Stephen J. Gould, Howard Sprecher and Horst Schulz. 2002. Functional characterization of $\Delta^{3}$,$\Delta^{2}$-Enoyl-CoA isomerases from rat liver. J. Biol. Chem. 277(11):9127-9132
  20. Bidanel, J. P., D. Milan, N. Iannuccelli, Y. Amigues, M. Y. Boscher, F. Bourgeois, J. C. Caritez, J. Gruand, P. Le Roy, H. Lagant, R. Quintanilla, C. Renard, J. Gellin, L. Ollivier and C. Chevalet. 2001a. Detection of quantitative trait loci for growth and fatness in pigs. Genet. Sel. Evol. 33(3):289-309


Supported by : National High Science and Technology Foundation of China