Effect of Additive on the Chemical Composition of Tra Catfish (Pangasius hypophthalmus) By-product Silages and Their Nutritive Value for Pigs

  • Thuy, Nguyen Thi (College of Agriculture and Applied Biology, Can Tho University) ;
  • Lindberg, Jan Erik (Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences) ;
  • Ogle, Brian (Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences)
  • Received : 2009.03.10
  • Accepted : 2009.07.30
  • Published : 2010.06.01


Two experiments were conducted to determine i) the fermentation characteristics of catfish by-product (C) ensiled with rice bran (RB) or sugarcane molasses (M) in different ratios, and ii) the digestibility of the silages in growing pigs. In the ensiling experiment, there were three ratios of C, ensiled with RB or M, of 8:2, 7:3 and 6:4 (wet basis for C and air-dry basis for RB and M). The six treatments were CRB8:2, CRB7:3, CRB6:4, CM8:2, CM7:3 and CM6:4, with 3 replications per treatment and seven sampling times. The pH of CRB7:3 and CRB6:4 decreased (p<0.05) from the first week and stayed stable until 8 weeks of ensiling, but the pH did not decrease in CRB8:2. The pH of CM8:2, CM7:3 and CM6:4 decreased rapidly from the first week until week 8. Dry matter (DM) and crude protein (CP) contents were slightly lower when catfish by-product was ensiled with RB than with M. Ammonia content in all treatments increased (p<0.05) during ensiling. Lactic acid content in silages with molasses increased from the first week, with the highest value at week 4. However, the lactic acid content in CRB8:2 did not increase with time and had the lowest value of all treatments. Acetic acid proportions of total volatile fatty acids were low at day 0, with the highest value at day 7, decreasing slowly until 8 weeks. The butyric acid proportion was highest at day 0 and decreased up to week 8. The proportion of propionic acid increased during ensiling. The digestibility experiment had a 4${\times}$4 Latin-square design and included 4 castrated crossbreed (Yorkshire${\times}$Landrace) male pigs fed four diets. The basal diet (BD) included rice bran, broken rice and maize meal, and the other three diets included fish meal (FMD) or catfish by-product ensiled with rice bran (CRBD) or sugarcane molasses (CMD). The silages chosen were those which gave the best results in the ensiling experiment for each additive. The coefficient of total tract apparent digestibility (CTTAD) of DM and organic matter (OM) was not significantly different among treatments. The CTTAD of CP was not different among the silage diets, but was lower in BD (p<0.01). The CTTAD of ether extract (EE) was higher in the catfish by-product silage diets than in FMD and BD. There was no significant difference in the digestibility of DM, OM, CP and EE between the fish meal and the by-product silages. In conclusion, catfish by-product can be successfully preserved by ensiling. Moreover, the total tract apparent digestibility of OM, CP and EE in ensiled catfish by-product was comparable with that of fish meal.


Supported by : Swedish International Development Cooperation Agency


  1. AOAC. 1990. Official methods of analysis. 15th edn. Association of Official Analytical Chemist., Washington DC. 1, pp. 69-90
  2. AOAC. 2000. Official methods of analysis of AOAC International. 17th edn. Vol. II, Chapter 32, pp. 47-49
  3. Cai, Y., S. Kumai, M. Ogawa, Y. Benno and T. Nakase. 1999. Characterization and identification of Pediococcus species isolated from forage crops and their application for silage preparation. Appl. Environ. Microbiol. 65:2901-2906
  4. Goddard, J. S. and J. S. M. Perret. 2005. Co-drying fish silage for use in aquafeeds. Anim. Feed Sci. Technol. 118:337-342
  5. Hassan, T. E. and J. L. Heath. 1987. Chemical and nutritive characteristics of fish silage produced by biological fermentation. Bio. Wastes 20:187-201
  6. Hong, T. T. T. and J. E. Lindberg. 2007. Effect of cooking and fermentation of a pig diet on gut environment and digestibility in growing pigs. Livest. Sci. 109:135-137
  7. Kaensombath, L. and B. Ogle. 2005. Laboratory-scale ensiling of Golden Apple snails (GAS)(Pomacea spp.). MSc thesis, Swedish University of Agricultural Sciences.
  8. Kung, L. and R. Shaver. 2001. Interpretation and use of silage fermentation analysis reports. Focus on Forage 3:1-5
  9. Lopez, C. S. 1989. Microbial ensilage of trash fish for animal feeds. In: Post-harvest technology, preservation and quality of fish in South - East Asia, (Ed. P. J. A. Reilly, R. W. H. Parry and L. E. Barile). International Foundation for Science, Stockholm pp. 189-191
  10. McDonald, P. 1981. The biochemistry of silage. John Wiley and Sons Ltd., Chichester, UK
  11. McDonald, P., R. A. Edwards, J. F. D. Greenhalgh and C. Morgan. 2002. Animal Nutrition, 6th Edition. Pearson Education Limited, Harlow Essex, UK. pp. 515-531
  12. McDonald, P., A. R. Henderson and S. J. E. Heron. 1991. The biochemistry of silage. Chalcombe Publications, Marlow, Bucks, UK
  13. Men, L. T., T. R. Preston, T. V. Hieu, D. T. Ngan and H. T. Loan. 2004. Evaluation of Tra catfish (Pangasius hypophthalmus) residue meal to replace fishmeal in diets for fattening pigs in the Mekong Delta of Vietnam. Livest. Res. Rural Dev.
  14. Mikkelsen, L. L. and B. B. Jensen. 1997. Effect of fermented liquid feed (FLF) on growth performance and microbial activity in the gastrointestinal tract of weaned piglets. Publication - European Assoc. for Anim. Prod. 88:639-642
  15. Muck, R. E. 1988. Factors influencing silage quality and their implications for management. J. Dairy Sci. 71:2992-3002
  16. Ngoan, L. D. and. J. E. Lindberg. 2000. Ensiling techniques for shrimp by-products and their nutritive value for pigs. Asian-Aust. J. Anim. Sci. 13:1278-1284
  17. Pahlow, G., R. E. Muck, F. Driehuis, S. J. Elferink and S. Spolestra. 2003. Microbiology of ensiling (Ed. D. R. Buxton, R. E. Muck and J. H. Harrison), Silage Sci. Technol. ASA Inc., Madison, WI, USA, 31-93
  18. Pettersson, K. L. and S. Lindgren. 1990. The influence of the carbohydrate fraction and additives on silage quality. Grass & Forage Sci. 45:223-233
  19. Phillips, M. 2002. Freshwater aquaculture in the lower mekong basin. MRC. Technical Paper No.7 Mekong River Commission, Phnom Penh. ISSN, pp. 1683-1489
  20. Phiny, C. and L. Rodr\'{\i}guez. 2001. Digestibility and nitrogen retention in Mong Cai pigs fed sugar palm (Borassus flabiller) juice and freshwater fish ensiled with rice bran and sugar palm syrup. Livest. Res. Rur. Dev. 13:2
  21. Prohaszka, L., B. M. Jayarao, A. Fabian and S. Kovacs. 1990. The role of intestinal volatile fatty acids in the Salmonella shedding of pigs. J. Vet. Med. Zentralblatt fuer Veterinaermedizin Reihe B. 37:570-574
  22. Rasool, S. and M. A. Sial. 1998. Chemical changes during ensiling of sudax fodder with broiler litter. Anim. Feed Sci. Technol. 72:347-354
  23. Rassol, S., S. Abrar and H. Gilani. 1996. Effect of yeast inoculation and molasses addition on ensiling sudax fodder with broiler litter. Indian J. Anim. Sci. 66:384-388
  24. Samuels, W. A., J. P. Fontenot, V. G. Allen and G. J. Flick. 1992. Fermentation characteristics of ensiled seafood wastes and low-quality roughages. Anim. Feed Sci. Technol. 38:305-317
  25. Schie, F. W. 1987. Some epidemiological and nutritional aspects of asymptomatic Salmonella infection in pigs. Ph.D. thesis. Faculty of Veterinary Medicine, University of Utrecht, Utrecht, Netherlands
  26. Scholten, R. H. J., C. M. C. van der Peet-Schwering, M. W. A. Verstegen, L. A. den Hartog, J. W. Schrama and P. C. Vesseur. 1999. Fermented co-products and fermented compound diets for pigs: a review. Anim. Feed Sci. Technol. 82:1-19
  27. Spackman, D. H., W. H. Stein and S. Moore. 1958. Automatic recording apparatus for use in chromatography of amino acids. Anal. Chem. 30:1190-1206
  28. Thuy, N. T., N. T. Loc, J. E. Lindberg and B. Ogle. 2007. Survey of the production, processing and nutritive value of catfish byproduct meals in the Mekong Delta of Vietnam. Livest. Res. Rur. Dev. 19, 124 19124.htm
  29. Tyrce, R. W., E. C. Clausen and J. L. Gaddy. 1991. The production of propionic acid from sugars by fermentation through lactic acid as an intermediate. J. Chem. Tech. Biotechnol. 50:157-166
  30. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597
  31. Wilder, M. and N. T. Phuong. 2002. The status of aquaculture in the Mekong Delta region of Vietnam: Sustainable production and combined farming systems. Proceedings of International Commemorative Symposium. Fisheries Sci. 68, Supplement I, November 2002
  32. Zahar, M., N. Benkerroum, A. Guerouali, Y. Laraki and K. El Yakoubi. 2002. Effect of temperature, anaerobiosis, stirring and salt addition on natural fermentation in silage of sardine and sardine wastes in sugarcane molasses. Bioresour. Technol. 82:171-176