Puberty Related Changes in Hormonal Levels, Productive Performance, Carcass Traits, and Their Interactions in Slovakian White Gilts

  • Kolesarova, A. (Slovak University of Agriculture, Department of Animal Physiology) ;
  • Sirotkin, A.V. (Animal Production Research Centre, Department of Genetics and Reproduction) ;
  • Roychoudhury, S. (Slovak University of Agriculture, Department of Animal Physiology) ;
  • Capcarova, M. (Slovak University of Agriculture, Department of Animal Physiology)
  • Received : 2009.05.12
  • Accepted : 2009.08.13
  • Published : 2010.02.01


The aim of this experiment was to evaluate the levels of hormones (progesterone, IGF-I and IGFBP-3) in blood plasma, growth, carcass traits and their interactions of sexually immature (n = 18) and sexually mature (n = 17) gilts. To calculate average daily weight gain (ADG), gilts were individually weighed at the beginning of the trial and at slaughter (110${\pm}$10 days old). Blood concentrations of progesterone, IGF-I and IGFBP-3 were determined by RIA. The right hot carcass sides were dissected and the individual basic parts from carcasses were weighed to record the carcass traits. IGFBP-3, ADG and carcass traits were not affected by pubertal maturation. Compared to sexually immature gilts, mature gilts had higher blood concentrations of progesterone and IGF-I. High correlations were noted between levels of some hormonal substances, productive performance and carcass traits of sexually immature and mature gilts.


  1. Al-Dabbas, F. M., A. H. Hamr and F. T. Awawdeh. 2008. The effect of arginine supplementation on some blood parameters, ovulation rate and concentrations of estrogen and progesterone in female Awassi sheep. Pakistan Journal of Biological Science 11:2389-2394
  2. Alfonso, L., J. Mourot, K. Insausti, J. A. Mendizabal and A. Arana. 2005. Comparative description of growth, fat deposition, carcass and meat quality characteristics of Basque and Large White pigs. Animal Research 54:33-42
  3. Arellano-Rodriguez, G., C. A. Meza-Herrera, R. Rodriguez- Martinez, R. Dionisio-Tapia, D. M. Hallford, M. Mellado and A. Gonzalez-Bulnes. 2008. Short-term intake of beta-carotenesupplemented diets enhances ovarian function and progesterone synthesis in goats. J. Anim. Physiol. Anim. Nutr. 2008 [Epub ahead o print]
  4. Arnhold, I. J., A. Lofrano-Porto and A. C. Latronico. 2009. Inactivating mutations of luteinizing hormone beta-subunit or luteinizing hormone receptor cause oligo-amenorrhea and infertility in women. Horm. Res. 71:75-82
  5. Barb, C. R., G. J. Hausman and C. A. Lents. 2008. Energy metabolism and leptin: effects on neuroendocrine regulation of reproduction in the gilt and sow. Reprod. Domest. Anim. 43: 324-330
  6. Blaszczyk, B., T. Stankiewicz, J. Udala and D. Gaczarzewicz. 2009. Plasma progesterone analysis by a time-resolved fluorescent antibody test to monitor estrous cycles in goats. Journal of Veterinary Diagnostic Investigations 21:80-87
  7. Budacova, A., A. V. Sirotkin, M. Kramarova, J. Kovacik, I. Florkovicova and P. Sanislo. 2001. Changes in function and responses of porcine ovarian granulosa cells during sexual maturation. Reprod. Abstr. Ser. 27:14
  8. Carter, A. M., K. Nygard, D. M. Mazzuca and V. K. Han. 2006. The expression of insulin-like growth factor and insulin-like growth factor binding protein mRNAs in mouse placenta. Placenta 27:278-290
  9. Davis, M. E. and R. C. Simmen. 2000. Genetic parameter estimates for serum insulin-like growth factor-I concentration and carcass traits in Angus beef cattle. J. Anim. Sci. 78:2305- 2313
  10. Galik, B., D. Biro, M. Juracek, M. Simko and J. Michalkova. 2007. Effect of combined biochemical additives on nutritive value and fermentation process of ensiled crimped high moisture corn. Slovak Journal of Animal Science 40:30-33
  11. Gardan, D., F. Gondret, K. Van Den Maagdenberg, N. Buys, S. De Smet and I. Louveau. 2008. Lipid metabolism and cellular features of skeletal muscle and subcutaneous adipose tissue in pigs differing in IGF-II genotype. Domest. Anim. Endocrinol. 34:45-53
  12. Hagan, C. R., E. J. Faivre and C. A. Lange. 2009. Scaffolding actions of membrane-associated progesterone receptors. Steroids 74:568-572
  13. Hutchison, M. R., M. H. Bassett and P. C. White. 2007. Insulinlike growth factor-I and fibroblast growth factor, but not growth hormone, affect growth plate chondrocyte proliferation. Endocrinology 148:3122-3130
  14. Istasse, L., C. Van Eenaeme, A. Gabriel, A. Clinquart, G. Maghuin- Rogister and J. M. Bienfait. 1990. The relationship between carcass characteristics, plasma hormones and metabolites in young fattening bulls. Vet. Res. Commun. 14:19-26
  15. Karamouti, M., P. Kollia, A. Kallitsaris, N. Vamvakopoulos, G. Kollios and I. E. Messinis. 2007. Growth hormone, insulin-like growth factor I, and leptin interaction in human cultured lutein granulosa cells steroidogenesis. Fertil. Steril. 90:1444-1450
  16. Krska, P., I. Bahelka, P. Demo and D. Peskovicova. 2002. Meat content in pigs estimated by various methods and compared with objective lean meat content. Czech Journal of Animal Science 47:206-211
  17. Langendijk, P., H. Van Den Brand, R. Gerritsen, H. Quesnel, N. Soede and B. Kemp. 2008. Porcine luteal function in relation to igf-1 levels following ovulation during lactation or after weaning. Reprod. Domest. Anim. 43:131-136
  18. Lee, C. Y., H. P. Lee, J. H. Jeong, K. H. Baik, S. K. Jin and J. H. Lee. 2002. Effects of restricted feeding, low-energy diet, and implantation of trenbolone acetate plus estradiol on growth, carcass traits, and circulating concentrations of insulin-like growth factor (IGF)-I and IGF-binding protein-3 in finishing barrows. J. Anim. Sci. 80:84-93
  19. Lei, M., X. Peng, M. Zhou, C. Luo, Q. Nie and X. Zhang. 2008. Polymorphisms of the IGF1R gene and their genetic effects on chicken early growth and carcass traits. BMC Genet. 9:70
  20. Lucy, M. C. 2008. Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reprod. Domest. Anim. 43:31-39
  21. Makarevich, A. V. and A. V. Sirotkin. 1999. Development of sensitive radioimmunoassay for IGF-I determination in samples from blood plasma and cell-conditioned medium. Vet. Med. (Prague) 44:71-78
  22. Makarevich, A. V., A. V. Sirotkin, T. Taradajnik and P. Chrenek. 1997. Effects of genistein and lavendustin on reproductive processes in domestic animals in vitro. J. Steroid Biochem. Mol. Biol. 63:329-337
  23. Nakatani, A., S. Shimasaki, G. F. Erickson and N. Ling. 1991. Tissue-specific expression of four insulin-like growth factorbinding proteins (1, 2, 3, and 4) in the rat ovary. Endocrinology 129:1521-1529
  24. Ong, K. K., M. Elmlinger, R. Jones, P. Emmett, J. Holly, M. B. Ranke and D. B. Dunger. 2007. Growth hormone binding protein levels in children are associated with birth weight, postnatal weight gain, and insulin secretion. Metabolism 56:1412-1417
  25. Ortega, H. H., M. M. Palomar, J. C. Acosta, N. R. Salvetti, B. E. Dallard and J. A. Lorente. 2008. Insulin-like growth factor I in sera, ovarian follicles and follicular fluid of cows with spontaneous or induced cystic ovarian disease. Res. Vet. Sci. 84:419-427
  26. Prunier, A. and I. Louveau. 1997. Influence of ovariectomy on metabolic and endocrine parameters during sexual development in the female pig. Journal of Endocrinology 154: 423-429
  27. Pursel, V. G., A. D. Mitchell, K. D. Wells, R. J. Wall, M. E. Coleman and R. J. Schwartz. 2000. Alteration of carcass composition in swine with an igf-i transgene. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, USA, January 9-12
  28. Roychoudhury, S., A. Kolesarova, J. Slivkova, P. Massanyi, A. Sirotkin and J. Bulla. 2009. Release of IGF-I by porcine ovarian granulosa cells after in vitro copper administration. Acta Fytotechnica et Zootechnica, Special Issue. 587-593
  29. Sirotkin, A. V., A. Benco, A. Tandlmajerova, D. Vasicek, J. Kotwica and K. Darlak. 2008. Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH. Reproduction 136: 611-618
  30. Sirotkin, A. V., R. Grossmann, M. T. Maria-Peon, J. Roa, M. Tena-Sempere and S. Klein. 2006. Novel expression and functional role of ghrelin in chicken ovary. Mol. Cell. Endocrinol. 257-258:15-25
  31. Sirotkin, A. V., P. Sanislo, H. J. Schaeffer, I. Florkovicova, J. Kotwica and J. Bulla. 2004. Thrombopoietin regulates proliferation, apoptosis, secretory activity and intracellular messengers in porcine ovarian follicular cells: involvement of protein kinase A. Journal of Endocrinology 183:595-604
  32. Sj$\ddot{o}$gren, K., J. O. Jansson, O. G. Isaksson and C. Ohlsson. 2002. A model for tissue-specific inducible insulin-like growth factor-I (IGF-I) inactivation to determine the physiological role of liver-derived IGF-I. Endocrine 19:249-256
  33. Staniar, W. B., D. S. Kronfeld, R. M. Akers and P. A. Harris. 2007. Insulin-like growth factor I in growing thoroughbreds. J. Anim. Physiol. Anim. Nutr. 91:390-399
  34. Sullivan, K. A., B. Kim and E. L. Feldman. 2008. Insulin-like growth factors in the peripheral nervous system. Endocrinology 149:5963-5971
  35. Xi, G., M. R. Hathaway, M. E. White and W. R. Dayton. 2007. Localization of insulin-like growth factor (IGFBP)-3 in cultured porcine embryonic myogenic cells before and after TGF-beta1 treatment. Domest. Anim. Endocrinol. 33:422-429
  36. Yang, G., J. Ren, S. Li, H. Mao, Y. Guo and Z. Zou. 2008. Genome-wide identification of QTL for age at puberty in female pigs using a large intercross F2 population between White Duroc×Erhualian. Genet. Sel. Evol. 40:529-539
  37. Yi, Z., M. R. Hathaway, W. R. Dayton and M. E. White. 2001. Effects of growth factors on insulin-like growth factor binding protein (IGFBP) secretion by primary porcine satellite cell cultures. J. Anim. Sci. 79:2820-2826