Effect of Exogenous Administration of Tamoxifen on Hormonal Profile and Sexual Maturity in Indian Native Kadaknath Fowl

  • Biswas, Avishek (Division of Physiology and Reproduction, Central Avian Research Institute) ;
  • Mohan, J. (Division of Physiology and Reproduction, Central Avian Research Institute) ;
  • Sastry, K.V.H. (Division of Physiology and Reproduction, Central Avian Research Institute)
  • Received : 2008.08.08
  • Accepted : 2009.01.19
  • Published : 2010.01.01


The effect of exogenous administration of tamoxifen (TAM) on hormonal profile and sexual maturity in Indian native Kadaknath (KN) fowl was investigated. Day-old chicks from the same hatch were randomly divided into 15 groups with 20 chicks in each group (5 treatments${\times}$3 replicates). The chicks were placed in battery brooders with wire-mesh floors and reared under uniform husbandry conditions (14 h light/d, 25-32${^{\circ}C}$) on a standard basal diet. At the age of two weeks (wk), birds from the control group ($T_{1}$) were injected with maize oil intramuscularly (I/M), whereas the other four experimental groups $T_{2}$, $T_{3}$, $T_{4}$ and $T_{5}$ were given tamoxifen (I/M) dissolved in maize oil at the rate of 0.5 mg (0.5 TAM), 1.0 mg (1.0 TAM), 2.5 mg (2.5 TAM) and 5.0 mg (5.0 TAM)/kg body weight, respectively, up to 30 wks on every alternate day. At every 6-wk interval, blood samples were collected from nine birds of each treatment group for estimation of estrogen and progesterone. The same birds were sacrificed for determination of the weight of ovary, oviduct, liver and adipose tissue. There was no significant difference in egg production traits except onset of lay and egg number. Low doses of TAM ($T_{3}$) advanced the onset of egg laying by 15 days over the control. Tamoxifen influenced the hormonal profile (estrogen and progesterone) in a dose dependent manner. However, higher doses of TAM suppressed ovary and oviductal growth. From this study, it may be concluded that lower doses of TAM enhanced sexual maturity while higher doses suppressed ovary and oviductal growth.


Kadaknath;Egg Production;Steroid Hormone;Ovary;Oviduct


  1. Aprahamian, S., M. J. Arslamin and J. K. Stoops. 1979. Effect of estrogen on fatty acid synthetase in the chicken oviduct and liver. Lipids. 14:1015-1020
  2. Duncan, B. 1955. Multiple range and multiple F tests. Biometrics. 11:1-12
  3. Etches, R. J. 1990. The ovulatory cycle of the hen. CRC Critical Rev. Poult. Biol. 2:293-318
  4. Lorenz, F. W. 1954. Effect of estrogen on domestic fowl and application in the poultry industry. Vitam. Horm. 12:235
  5. Swain, B. K., T. S. Johari and S. Majumder. 2000. Effect of supplementation of vitamin E and selenium and their different combination on the performance and immune response of broilers. Br. Poult. Sci. 41:287-292
  6. Rozenboim, I., G. Gvaryahu, B. Robinzon, N. Sayag and I. Snapir. 1986. The induction of precocious development of reproductive function in cockerels by tamoxifen administration. Poult. Sci. 65:1980-1983
  7. Lin, Y. F., S. J. Chang, J. R. Yang, Y. P. Lee and A. L. Hsu. 2005. Effect of supplemental vitamin E during the mature period on the reproductive performance of Taiwan native chicken cockerels. Br. Poult. Sci. 46:366-372
  8. Rozenboim, I., I. Snapir, E. Arnon, R. Argych, W. H. Bucken, P. J. Sharp, Y. Koch and B. Robinzon. 1993. Precocious puberty in tamoxifen treated cockerels; hypothalamic gonadotropin releasing hormone, plasma LH prolactin growth hormone and testosterone. Br. Poult. Sci. 34:533-542
  9. Rozenboim, I., O. Dgany, B. Robinzon, E. Arnon and I. Snapir. 1989. The effect of tamoxifen on the reproductive traits in white leghorn cockerels. Pharm. Biochem. Behav. 32:377-381
  10. Jaccoby, S., N. Snapir, E. Rozenboim, E. Arnon, R. Meidan and B. Robinzon. 1992. Tamoxifen advances puberty in the white leghorn. Br. Poult. Sci. 33:101-111
  11. Hafez, E. S. E. 1995. Reproduction in farm animals. 6th Edition, Len and Febiger, Philadelphia, USA
  12. Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods. 8th Edition. Oxford and IBH Pub. Co., Kolkata, India
  13. Wilson, S. C., P. G. Knight and F. J. Cunnigham. 1983. Evidence for the involvement of central conversion of testosterone to estradiol-17$\beta$ in the regulation of LH secretion in the cockerel. J. Endocrinol. 99:301-310
  14. Surai, P. F. 2002. Selenium on poultry nutrition, antioxidants properties, deficiency and toxicity. World's Poult. Sci. J. 58:333-364
  15. Snapir, N., B. Robinzon and B. Shalita. 1983. The involvement of gonads and gonadal steroids in the regulation of feed intake, body weight, and adiposity in the white leghorn cock. Pharm. Biochem. Behav. 19:617-624