Effect of Quercetin on the Activity and mRNA Expression of Antioxidant Enzymes and Physiological Responses in Olive Flounder (Paralichthys olivaceus) Exposed to Cadmium

  • Shin, H.S. (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Yoo, J.H. (Jeilfeed company Ltd.) ;
  • Min, T.S. (Division of Medical and Pharceutical Science, National Research Foundation of Korea) ;
  • Lee, J. (Department of Marine Life Sciences, Jeju National University) ;
  • Choi, C.Y. (Division of Marine Environment and Bioscience, Korea Maritime University)
  • Received : 2010.01.05
  • Accepted : 2010.03.24
  • Published : 2010.06.01


We investigated the antioxidant efficacy of quercetin (0% Diet 1, 0.25% Diet 2, and 0.5% Diet 3) pretreatment for 30 and 60 days in response to cadmium (Cd) toxicity in the olive flounder, and measured the plasma lysozyme activity to understand the immune effects of quercetin. The lysozyme activity with Diets 2 and 3 was higher than with Diet 1. Based on this result, to examine the immune ability and antioxidant role of quercetin, we exposed olive flounder fed quercetin to Cd and then measured the expression and activity of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) and lipid peroxidation (LPO). With Diets 2 and 3, the expression and activity of antioxidant enzymes and the $H_2O_2$ concentration were lower than with Diet 1. In addition, the LPO levels were lower than with Diet 1, which protected the cell membrane. Therefore, quercetin removed the reactive oxygen species (ROS) produced by Cd, indicating that quercetin has antioxidant ability. In addition to its antioxidant ability, quercetin has immune effects.


Supported by : NIPA (National IT Industry Promotion Agency)


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