Effect of BTS and Androhep during Storage Times on the Kinematics and Capacitation Status in Liquid Boar Semen

BTS와 Androhep이 보존 기간 동안 액상 정액의 운동역학 및 수정능 획득에 미치는 영향

  • Kim, Yun-Hee (Department of Animal Science and Technology, Chung-Ang University) ;
  • Park, Yoo-Jin (Department of Animal Science and Technology, Chung-Ang University) ;
  • Yoon, Sung-Jae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kwon, Woo-Sung (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Sang-Hyun (Darby Pig AI Center) ;
  • Pang, Myung-Geol (Department of Animal Science and Technology, Chung-Ang University)
  • 김연희 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 박유진 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 윤성재 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 권우성 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 김상현 ((주)다비AI센터) ;
  • 방명걸 (중앙대학교 산업과학대학 동물자원과학과)
  • Received : 2010.09.13
  • Accepted : 2010.09.17
  • Published : 2010.09.30

Abstract

The objective of this study was to investigate the effect of storage time on fresh boar semen in Androhep and Beltsville Thawing Solution (BTS). Boar semen samples extended in each extender were stored at $17^{\circ}C$ up to 4 days. Sperm motility kinematics was evaluated by computer assisted sperm analyzer (CASA) and capacitation status by chlortetracycline (CTC)/Hoechst 33258 staining. Sperm motility (%) was not decreased during storage in BTS and Androhep. No significant difference between extenders was observed. Only significant differences in kinematic parameters on linearity during storage were found. The percentage of dead sperm significantly decreased during storage (p<0.05). Also the percentage of noncapacitated, capacitated, and acrosome-reacted sperm significantly modified during storage (p<0.05). However, there was no significant difference between extenders except proportion of capacitated sperm. This finding supported that modification in these parameters was not significantly different between extenders during this short-term storage. Our finding strongly indicated that both Androhep and BIS maintained favorable conditions for motility, motility kinematics, and capacitation status during short-term storage. Despite modifications in some parameters were apparent during sperm storage in extenders, these may not affect the fertilizing capacity of boar semen.

Acknowledgement

Supported by : 농촌진흥청

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