Genetic Distinctness of the Korean Red-backed Vole (Myodes regulus) from Korea, Revealed by the Mitochondrial DNA Control Region

  • Received : 2010.04.29
  • Accepted : 2010.11.12
  • Published : 2010.11.30


To identify Korean red-backed voles (Myodes regulus) from Korea by mitochondrial DNA (mtDNA) sequencing, we obtained mtDNA control region sequences of 17 red-backed voles from Korea and northeast China, and these sequences were compared with the corresponding haplotypes of Myodes obtained from GenBank. We identified five red-backed voles from Mt. Changbai and Harbin as M. rufocanus and another three redbacked voles from Harbin as M. rutilus, respectively. Moreover, nine red-backed voles from Korea, showing the average nucleotide distance of 0.66% among nine haplotypes, were different from other species of Myodes, and the average distance between nine haplotypes of red-backed voles from Korea and seven haplotypes of M. rufocanus was 6.41%, whereas the average distance between nine haplotypes of red-backed voles from Korea and five haplotypes of M. rutilus was 14.8%. We identified the red-backed voles from Korea as M. regulus, and found that M. regulus is distinct in its mtDNA control region sequences as well, although we propose further analyses with additional specimens from East Asia using nuclear and mtDNA markers to confirm the distinctness of M. regulus.


  1. Cook, J.A., A.M. Runck and C.J. Conroy, 2004. Historical biogeography at the crossroads of the northern continents: molecular phylogenetics of red-backed voles (Rodentia: Arvicolinae). Mol. Phylogenet. Evol., 30: 767-777.
  2. Corbet, G.B., 1978. The Mammals of the Palaearctic region: a taxonomic review. British Mus. (Nat. Hist.), Cornell Univ. Press, London, pp. 97-103.
  3. Huelsenbeck, J.P., J.J. Bull and C.W. Cunningham, 1996. Combining data in phylogenetic analysis. TREE, 11: 152-157.
  4. Iwasa, M.A., I.V. Kartavtseva, A.K. Dobrotvorsky, V.V. Panov and H. Suzuki, 2002. Local differentiation of Clethrionomys rutilus in northeastern Asia inferred from mitochondrial gene sequences. Mamm. Biol., 67: 157-166.
  5. Iwasa, M.A., S.H. Han and H. Suzuki, 1999. A karyological analysis of the Korean red-backed vole, Eothenomys regulus (Rodentia, Muridae), using differential staining methods. Mammal Study, 24: 35-41.
  6. Iwasa, M.A., Y. Utsumi, K. Nakata, I.V. Kartavtseva, I.A. Nevedomskaya, N. Kondoh and H. Suzuki, 2000. Geographic patterns of cytochrome b and Sry gene lineages in the grey red-backed vole Cletrionomys rufocanus from far east Asia including Sakhalin and Hokkaido. Zoological Science, 17: 477-484.[477:GPOCBA]2.0.CO;2
  7. Iwasa, M.A. and H. Suzuki, 2002. Evolutionary significance of chromosome changes in northeastern Asiatic red-backed voles inferred with the aid of intron 1 sequences of the G6pd gene. Chromosome Research, 10: 419-428.
  8. Jones, J.K. and D.H. Johnson, 1965. Synopsis of the lagomorphs and rodents of Korea. Univ. Kansas Publ. Mus. (Nat. Hist.), 16: 357-407.
  9. Kaneko, Y., 1990. Identification and some morphological characters of Clethrionomys rufocanus and Eothenomys regulus from USSR, northeast China, and Korea in comparison with C. rufocanus from Finland. J. Mamm. Soc. Japan, 14: 129-148.
  10. Kumar, K., K. Tamura and M. Nei, 2004. MEGA3: Integrated software for molecular evolutionary analysis and sequence alignment. Briefings in Bioinformatics, 5: 150-163.
  11. Mallet, J. and K. Willmott, 2003. Taxonomy: renaissance or Tower of Babel? TREE, 18: 57-59.
  12. Musser, G.G. and M.D. Carleton, 2005. Order Rodentia. In: Mammal species of the world: a taxonomic and geographic reference (Eds., D.E. Wilson and D.M. Reeder). pp. 1020-1029. The Johns Hopkins University Press, Baltimore.
  13. Quicke, D.L.J., 1993. Principles and techniques of contemporary taxonomy. Blackie Academic & Professional, New York, 311pp.
  14. Rubinoff, D. and B.S. Holland, 2005. Between two extremes: mitochondrial DNA is neither the panacea nor the nemesis of phylogenetic and taxonomic inference. Syst. Biol., 54: 952-961.
  15. Shields, G.F. and T.D. Kocher, 1991. Phylogenetic relationships of North American ursids based on analysis of mitochondrial DNA. Evolution, 45: 218-221.
  16. Sunnucks, P., 2000. Efficient genetic markers for population biology. TREE, 15: 199-203.
  17. Suzuki, H., M. Iwasa, M. Harada, S. Wakana, M. Sakaizumi, S.H. Han, E. Kitahara, Y. Kimura, I. Kartavtseva and K. Tsuchiya, 1999. Molecular phylogeny of red-backed voles in far east Asia based on variation in ribosomal and mitochondrial DNA. J. Mamm., 80: 512-521.
  18. Tamura, K. and M. Nei, 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol., 10: 512-526.
  19. Wakana, S., M. Sakaizumi, K. Tsuchiya, M. Asakawa, S.H. Han, K. Nakata and H. Suzuki, 1996. Phylogenetic implications of variations in rDNA and mtDNA in red-backed voles collected in Hokkaido, Japan, and in Korea. Mammal Study, 21: 15-25.
  20. Zhang, Y., J. Sangke, J. Guoqiang, L. Shibau, Y. Zhongyao, W. Genggui and Z. Manli, 1997. Distribution of mammalian species in China. China Forestry Publishing House, Beijing, pp. 227-233.

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