Development of Anti-Wrinkle Agent from Nelumbo nucifera Root Extract

연근 추출물에서 주름개선 소재의 개발

Kim, Hee Jin;Kim, Tagon;Kang, Whan Yul;Baek, Hyun;Cheon, Hae Young;Kim, Bo Young;Kim, Donguk
김희진;김타곤;강환열;백현;전혜영;김보영;김동욱

  • Received : 2010.02.22
  • Accepted : 2010.03.24
  • Published : 2010.08.31

Abstract

In this research, root extracts of Nelumbo nucifera was tested to see the possibility for functional cosmetic agent. 70-100% ethanol was used as solvent and nuciferin was confirmed as active component. To test cosmetic effect of root extracts of Nelumbo nucifera, safety effect(MTT assay), anti-wrinkle effect(elastase inhibition assay) and antioxidation effect(DPPH free radical scavenging assay) were measured. When 100% ethanol was used as extracting solvent, cell viability was over 80% at $100{\mu}g/ml$, which indicated that root extract of Nelumbo nucifera was suitable for cosmetic agent. Root extract of Nelumbo nucifera showed 40~50% elastase inhibition at $100{\mu}g/ml$ so that it had good anti-wrinkle characteristics. 50% antioxidation capacity($FSC_{50}$) was $5.0{\sim}38{\mu}g/ml$ and root extract of Nelumbo nucifera showed excellent antioxidation effect. From the research, root extracts of Nelumbo nucifera showed strong possibility for anti-wrinkle functional cosmetic agent.

Keywords

Nelumbo Nucifera Root;Ethanol Extraction;Anti-Wrinkle;Antioxidation

References

  1. KDA Textbook Editiong Board, Dermatology, 5th ed., Ryo Mook Gak, Seoul(2008).
  2. Korea Food and Drug Administration, http://www.kfda.go.kr/index.html.
  3. Elsner, P. and Mailbach, H. I., Cosmeceuticals and Active Cosmetics, 2nd ed., Taylor & Francis, New York, NY(2005).
  4. Lee, K. H. and Park, S. N., "Antioxidative Activities and Antiaging Effects of Geranium nepalense Extracts," J. Soc. Cosmet. Scientists Korea, 34(1), 25-35(2008).
  5. Yang, H. J. and Park, S. N., "Component Analysis and Study on Anti-elastase Activity of Equisetum arvense Extracts (II)," J. Soc. Cosmet. Scientists Korea, 33(3), 139-144(2007).
  6. Sim, G. S., Kim, J. H., Lee, D. H., Lee, B. C., Lee, G. S. and Pyo, H. B., "The Inhibition fo UVA-induced Matrix Metalloproteinase-1 in Human Dermal Fibroblasts and the Improvement of Skin Elasticity by Cirsium setidens Extract," J. Soc. Cosmet. Scientists Korea, 33(3), 181-187(2007).
  7. Kim, D. H., Choi, H. K., Cho, S. C., Kook, M. C. and Park, C. S., "Enhancement of Antioxidant and Anti-aging Activities fo Spirulina Extracts," J. Soc. Cosmet. Scientists Korea, 34(3), 225-231(2008).
  8. Pharmacognosy Researchers, Modernpharmacognosy, Hak Chan Publishing, Seoul(2000).
  9. Park, J. C., Functional Food and Oriental Medicine, Hyoilbooks, Seoul(2007).
  10. Association of Official Analytical Chemists, A.O.A.C., Official Methods of Analysis, 15th ed., Washington DC.(1990).
  11. Mosmann, T., "Rapid Colorimetric Assay for the Cellular Growth and Survival Application to Proliferation and Cytotoxic Assay," J. Immun Method, 65, 55-62(1983). https://doi.org/10.1016/0022-1759(83)90303-4
  12. Kim, H. J., "Development of Functional Cosmetic Agent from Nelumbo nucifera," Master Thesis, Inje University, Gimhae, Gyongnam(2009).
  13. Park, S. K., Hong, S.-K., Kim, H. J., Kim, B. Y., Kim, T. G., Kang, J. S. and Kim, D., "Cosmetic Effect of Angelica gigas Nakai Root Extracts," Korean Chem. Eng. Res., 47, 553-557(2009).
  14. Kim, J. Y., Yang, H. J., Lee, K. H., Jeon, S. M., Ahn, Y. J., Won, B. R. and Park, S. N., "Antioxidative and Antiaging Effects of Jeju Native Plant Extracts (II)," J. Soc. Cosmet. Scientists Korea, 33(3), 165-173(2007).

Acknowledgement

Supported by : 한국산업기술평가원