Standardization of Ecotoxicity Assay Method for Heavy Metals using Inhibition of Dehydrogenase Activity

탈수소효소 활성도 저해를 이용한 중금속 생태독성 평가법의 표준화

  • Oh, Kyoung-Hee (Department of Environmental Engineering, Chungbuk National University) ;
  • Han, Ah-Won (Department of Environmental Engineering, Chungbuk National University) ;
  • Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
  • 오경희 (충북대학교 공과대학 환경공학과) ;
  • 한아원 (충북대학교 공과대학 환경공학과) ;
  • 조영철 (충북대학교 공과대학 환경공학과)
  • Received : 2009.07.14
  • Accepted : 2010.06.03
  • Published : 2010.07.30

Abstract

In this study the enzyme inhibition method using dehydrogenase which has been popularly used to estimate ecotoxicity was optimized. When three bacterial strains, Escherichia coli HB101, Enterobacter asburiae KCAD-4, and Aeromonas media KCAD-13, were compared, KCAD-4 was considered as the adequate strain to estimate toxicity because of its sensitivity and reproducibility. The optimal bacterial density was estimated as $5.4{\times}10^9CFU/mL$, at which the maximum sensitivity was observed. The phosphate buffer was suitable for the reaction solution. When the reaction times required for inhibition of enzyme activity by contact of toxicants and for reaction of damaged bacteria and substrate were tested, the optimal value was estimated as 20 min and 2 hrs, respectively. It is expected that the optimized conditions can be used to develop the standardized kits to estimate ecotoxicity of heavy metals in effluent from the industrial wastewater treatment facilities.

Keywords

Acknowledgement

Supported by : 충북대학교

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