Anti-inflammatory Effect of Artemisia Capillaris Thunberg in Lipopolysaccharide-exposed Rats

인진호(茵蔯蒿)가 LPS 염증유발 흰쥐의 전염증성 cytokine 생산 및 혈액성상에 미치는 영향

  • Seo, Yong-Seok (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sang-Ji University) ;
  • Lee, Eun (College of Health Science, Sang-Ji University) ;
  • Cha, Yun-Yeop (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sang-Ji University)
  • 서용석 (상지대학교 한의과대학 한방재활의학과교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 차윤엽 (상지대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.06.14
  • Accepted : 2010.07.14
  • Published : 2010.07.30

Abstract

Objectives : The present study investigated anti-inflammatory effect of Artemisia Capilaris Thunberg in lipopolysaccharide-exposed rats. Methods : We divided lipopolysaccharide-exposed Sprague-Dawley rats into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 6 weeks. We measured counts of red blood cell(RBC), the values of hemoglobin(Hb) and packed cell volume(PCV), plasma total protein concentration, albumin concentration, the ratio of albumin/globulin, the activities of plasma glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT), lactate dehydrogenase(LDH), the counts of white blood cell(WBC), the ratio of neutrophils, lymphocytes, monocytes, basophils, eosinophils, the concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma interleukin-6(IL-6), plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$), plasma interleukin-10(IL-10), the concentration of liver $IL-1{\beta}$ and IL-6, $TNF-{\alpha}$, IL-10. Results : Counts of RBC and the values of Hb and PCV, plasma total protein concentration and albumin concentration, the activities of plasma GOT, GPT and LDH showed no significant difference in the treatment groups. and the ratio of albumin/globulin was increased in Artemisia Capillaris Thunberg groups. The counts of WBC showed lower values in Artemisia Capillaris Thunberg groups than those of control group, In the ratio of neutrophils Thunberg groups. The ration of monocytes, basophils and eosinophils were below 5%, and showed no characteristic trend. The concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma inerleukin-6(IL-6) and plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$) showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, and the concentration of plasma interleukin-10(IL-10) showed no significant difference in the treatment groups. The concentration of liver $IL-1{\beta}$ and IL-6 showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, however the concentration of liver $TNF-{\alpha}$ and IL-10 showed no significant difference in the treatment groups. Conclusions : The Artemisia Capillaris Thunberg groups gives positive results of anti-inflammatory response by lipopolysaccharide(LPS) derivation.

Keywords

Artemisia Capillaris Thunberg;Anti-inflammatory effect;Cytokine

Acknowledgement

Supported by : 상지대학교

References

  1. 김영홍, 정미영, 이나경, 이진용, 허익, 이제현, 임사비나. 韓茵蔯의 치주염세균에 대한 항균효과 및 항염효과. 대한본초학회지. 2008;23(2):1-8.
  2. A. rabson, IM. Roitt, PJ. Delves. Really essential medical immunology. Oxford:Blackwell publishing Ltd. 2005:1-14.
  3. Lee SJ. Studies on the origin of korean folk medicines(1). Kor J Phamacognosy. 1975;6(2):75-93.
  4. Huh J. Book of oriental medicine. Seoul: Namsan-dang publishing Co. 1976:362-70.
  5. Chao CY, SL Yeh, MT Lin, WJ Chen. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids and inflammatory mediators in septic rats. Nutrition. 2000;16:284-8. https://doi.org/10.1016/S0899-9007(99)00299-3
  6. 노영득, 심영섭, 송호준, 신민교. 목단피의 항염 효과에 관한 연구. 대한본초학회지. 2004;19(3):13-24.
  7. 변성희, 양재하, 김상찬. 현삼메탄올 추출물이 LPS로 유도된 Raw 264.7 cell에서의 $TNF-\alpha$, $IL-1\beta$, IL-6 및 nitric oxide 생성에 미치는 영향. 대한본초학회지 2005;20(2):7-16.
  8. 장선일, 김형진, 황기명. 활성화된 설치류 Raw 264.7 대식세포에서 당귀에탄올 추출물의 항염 증효과. 대한방제학회지. 2002;10(2):189-97.
  9. 박용기, 정효원, 김창민, 최재수, 김영식. 황련의 주성분인 Berberine의 신경소교세포로부터 LPS에 의해 유도되는 염증매개물질 생성억제효과. 대한본초학회지. 2007;22(4):117-25.
  10. 정용래, 이승덕, 변혁, 박인식, 정찬영, 이창환, 김갑성. 녹용약침이 LPS 유발 관절염의 윤활 관절막내 과다염증반응 조절에 미치는 영향. 대한침구학회지. 2007;24(4):167-81.
  11. 박기범, 백승태, 이승덕, 김경호, 김갑성. 자하거 약침의 항염증능이 LPS유발 관절염치료에 미치는 영향. 대한침구학회지. 2006;23(6):103-15.
  12. Choi WS, Kim CJ, Park BS, Lee SE, Takeoka GR, Kim DG, Lanpiao X, Kim JH. Inhibitory effect on proliferation of vascular smooth muscle cells and protective effect on CCL(4)-induced hepatic damage of HEAI extract. J Ethnopharmacol. 2005;100(1-2):176-9. https://doi.org/10.1016/j.jep.2005.02.037
  13. Gilani AH, Yaeesh S, Jamal Q, Ghayur MN. Hepatoprotective activity of aqueous-methanol extract of Artemisia vulgaris. Phytother RES. 2005;19(2):170-2. https://doi.org/10.1002/ptr.1632
  14. Kim KS, Lee S, Lee YS, Jung SH, Park Y, Shin KH, Kim BK. Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea. J Ethnopharmacol. 2003;85(1):69-72. https://doi.org/10.1016/S0378-8741(02)00338-0
  15. Yin J, Wennberg RP, Miller M. Induction of hepatic bilirubin and drug metabolizing enzymes by individual herbs present in the traditional Chinese medicine, yin zhi huang. Dev Pharmacol Ther. 1993;20(3-4):186-94. https://doi.org/10.1159/000457561
  16. Seong Yeob Kang, Sung Hoon Kim, Su Myung Kim, Uk Namgoong, Dong Hee Kim. Effect of Artemisiae capillaris herba on anti-inflammatory properties in RAW264.7 cell line. Korean J. Oriental Physiology & Pathology. 2004;18(6):1832-42.
  17. Mathiak G, G Grass, T Herzmann, T Luebke, C Cu-Zetina, SA Boehm. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000;131:383-6. https://doi.org/10.1038/sj.bjp.0703629
  18. 김현제. 한의학사전. 서울:성보사. 1988:194.
  19. 문류모. strees에 관한 문헌적 고찰. 동의신경정신과학회지. 1991;2(1):38-50.
  20. Chamulitrat W, ME Blazka, SJ Jordan, MI Luster, RP Mason. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995;24:2273-80.
  21. Harbrecht BG, M DiSilvio, AJ Demetris, RL Simmons, TR Billiar. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994;20:1055-60. https://doi.org/10.1002/hep.1840200439
  22. Hamada E, T Nishida, Y Uchiyama, J Nakamura, K Isihara, H Kazuo. Activation of kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J. Hepatol. 1999;30:807-18. https://doi.org/10.1016/S0168-8278(99)80133-0
  23. Marriot JB, M Westby, S Cookson, M Guckian, S Goodbourn, G Muller. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-$\alpha$ production. J. Immunol. 1998;161:4236-43.
  24. Tompson KC, A Trowern, A Fowell, M Marathe, C Haycock, MJ. Arthur, et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998;28:1518-24. https://doi.org/10.1002/hep.510280611
  25. Moreira AL, EP Sampaio, A Zmuidzinas, P Frindt, KA Smith, G Kaplan. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J. Exp. Med. 1993;177:1675-80. https://doi.org/10.1084/jem.177.6.1675