Effects of Imyo-san Treatment on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

이묘산(二妙散)이 흰쥐의 Monosodium Iodoacetate 유발 골관절염에 미치는 영향

  • Ahn, Hee-Bin (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Soon-Jooog (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Se-Myung University) ;
  • Jeong, Su-Hyeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University)
  • 안희빈 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 정수현 (세명대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.06.15
  • Accepted : 2010.07.13
  • Published : 2010.07.30

Abstract

Objectives : This study was to investigate the effects of Imyo-san treatment on the monosodium iodoacetate-induced osteoarthritis in rats. Methods : Arthritis was induced by injection of monosodium iodoacetate(MIA)(0.5 mg) into both knee joint cavities of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken distilled water and treated group was taken extracts of Imyo-san by orally for 20 days. At the end of experiment(20day after MIA injection), gross and histopathological examination on the articular structures of knee joints were performed. Blood cell counts and proteoglycan(PG) contents in articular cartilages were analysed. And also, tumor necrosis factor-$\alpha$($TNF-{\alpha}$) and interleukin-$1{\beta}$($IL-1{\beta}$) contents synovial fluids were measured by enzyme-liked immunosorbent assay(ELISA) method. Results : 1. Body weight(g) of the treated group were increased significantly compared with control group at 15 and 20 days after injection. 2. Grossly, degree of osteoarthritis in the treated group was alleviated compared with the control group. 3. PG content in articular cartilage of the treated group was increased significantly compared with the control group. 4. Histopathologically, osteoarthritic scores of the treated group was decreased significantly compared with the control group. 5. $TNF-{\alpha}$ content in synovial fluid of the treated group was decreased significantly compared with the control group. Conclusions : On the basis of these results, we suggested that Imyo-san has inhibiting effects on the progression of arthritis in MIA-induced osteoarthritis model.

Keywords

Imyo-san;Osteoarthritis;Proteoglycan;$TNF-{\alpha}$

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