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Bioconversion of Ginsenoside Rd into Compound K by Lactobacillus pentosus DC101 Isolated from Kimchi

  • Quan, Lin-Hu (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Cheng, Le-Qin (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Ho-Bin (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Ju-Han (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Son, Na-Ri (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Se-Young (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Jin, Hyun-O (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University)
  • Received : 2010.07.02
  • Accepted : 2010.08.28
  • Published : 2010.12.29

Abstract

Ginsenosides are the principal components responsible for the pharmacological and biological activities of ginseng. Ginsenoside Rd was transformed into compound K using cell-free extracts of food microorganisms, with Lactobacillus pentosus DC101 isolated from kimchi (traditional Korean fermented food) used for this conversion. The optimum time for the conversion was about 72 h at a constant pH of 7.0 and an optimum temperature of about $30^{\circ}C$. The transformation products were identified by thin-layer chromatography and high-performance liquid chromatography, and their structures were assigned using nuclear magnetic resonance analysis. Generally, ginsenoside Rd was converted into ginsenoside F2 by 36 h post-reaction. Consequently, over 97% of ginsenoside Rd was decomposed and converted into compound K by 72 h post-reaction. The bioconversion pathway to produce compound K is as follows: ginsenoside Rd$\rightarrow$ginsenoside F2$\rightarrow$compound K.

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