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Isolation and Identification of a Biphenyl-degrading Bacterium, Pseudomonas sp. DS-94

Biphenyl 분해 미생물 Pseudomonas sp. DS-94의 분리 및 동정

  • Lee, Dae-Sung (POSTECH Ocean Science & Technology Institute, POSTECH) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu)
  • 이대성 (포항공과대학교 해양대학원) ;
  • 정성윤 (대구가톨릭대학교 의생명과학과)
  • Received : 2010.09.04
  • Accepted : 2010.10.12
  • Published : 2010.11.30

Abstract

Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were $25^{\circ}C$ and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.

Keywords

Biphenyl-degrading activity;Contaminated soil;Pseudomonas sp. DS-94

Acknowledgement

Supported by : 한국연구재단

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