Comparisons of Estrus Induction with Anti-Prolactin and Hormones in Bitches

Anti-Prolactin과 다양한 호르몬 투여에 따른 개의 발정 유도 효율 비교

  • Heo, Young (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Eun-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Maeng, Geun-Ho (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Min-Jung (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jo, Gyu-Wan (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Sung-Lim (College of Veterinary Medicine, Gyeongsang National University)
  • 허영 (경상대학교 수의과대학 수의학과) ;
  • 강은주 (경상대학교 수의과대학 수의학과) ;
  • 맹근호 (경상대학교 수의과대학 수의학과) ;
  • 김민정 (경상대학교 수의과대학 수의학과) ;
  • 조규완 (경상대학교 수의과대학 수의학과) ;
  • 이성림 (경상대학교 수의과대학 수의학과)
  • Published : 2009.09.30


Domestic bitches are non-seasonally monoestrus; spontaneously ovulate only once or twice occurs at anytime of the year. Estrus induction has been applied infrequent estrus, misleading ovulation, mating difficulties, failure to conceive after normal mating, pregnancy failure and biological research. Protocol of estrus induction which included variable hormones such as FSH, GnRH, and PMSG have been applied for the last decades. Recently, Bromocriptine, one of anti-prolatin/dopamine agonist has been occasionally applied for estrus induction. The study was carried out to investigate the effective method for the induction of estrus in bitches using different hormone treatments, and the initiation time of estrus from hormone treatment by assessments of cytological observation and blood plasma progesterone concentration. A total of 54 bitches on anestrus were selected for the study and divided randomly into 8 treatment groups as follow. Control, natural estrus; FSH (L), FSH (1.5 mg/kg, twice a day, $Falltrophin^{(R)}$, Vetrepharm); FSH (H), FSH (3.0 mg/kg, twice a day); GnRH+FSH, GnRH (5 ug/kg, once first day, $GNADON^{(R)}$, Dongbang)+FSH (3 mg/kg, SID); PMSG, PMSG (50 IU/kg, every third day $FOLLIGON^{(R)}$, Intevet); GnRH+PMSG, GnRH (5 ug/kg, only first day)+PMSG (50 IU/kg, every third day); GnRH, GnRH (5 ug/kg, only first day); Bromocriptine, bromocriptine (0.3 mg/kg, SID, $Parlodel^{(R)}$, Novartis). The bitches were evaluated clinical sign, cytological exam and $OVUCHECK^{(R)}$ Premate for assessment of estrus induction. Estrus induction rates were significantly (P<0.05) higher in GnRH+PMSG (100%) compared to others. PMSG and GnRH+PMGS (87.5 and 100%) and Bromocriptine (77.8%) were higher than others except GnRH+PMSG. Analysis of vaginal smear has proved to be effective a correct assessment of estrus induction with assay of progesterone concentration by $OVUCHECK^{(R)}$ Premate. Proestrus initiated by the $6^{th}$ after induction in most case. In conclusion, bromocriptine is an effective drug for estrus induction in bitches and assay of progesterone concentration by $OVUCHECK^{(R)}$ Premate with examination of vaginal smear that should be useful to detection of estrus induction of estrus induced bitches.


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