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Simultaneous Determination of Tetracyclines and Sulfonamides Residues in Pork and Flatfish Using Matrix Solid Phase Dispersion (MSPD) Extraction and HPLC

MSPD와 HPLC를 이용한 돼지고기 및 광어 중의 테트라싸이클린계 항생제와 sulfonamide의 다성분 잔류분석법 개발

  • Gil, Geun-Hwan (National Academy of Agricultural Science, RDA) ;
  • Ko, Kwang-Yong (Dept. of Agriculture Chemistry, Chungnam National University) ;
  • Lee, Yong-Jae (Central Research Institute, KT&G) ;
  • Park, Hye-Jin (Food Science Department South Dakota State University) ;
  • Lee, Kyu-Seung (Dept. of Agriculture Chemistry, Chungnam National University)
  • 길근환 (농촌진흥청 국립농업과학원) ;
  • 고광용 (충남대학교 농화학과) ;
  • 이용재 (KT&G 중앙연구원) ;
  • 박혜진 (사우스다코타 주립대학교 식품화학부) ;
  • 이규승 (충남대학교 농화학과)
  • Published : 2009.09.30

Abstract

This study was conducted to develop more convenient simultaneous determination method by matrix solid phase dispersion (MSPD) and HPLC for sulfonamides such as sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfaquinoxaline (SQX), and tetracyclines including oxytetracycline (OTC), tetracycline (TC) chlortetracycline (CTC) in prok and flatfish. The limits of detection were 0.047 $mg{\cdot}kg^{-1}$ for OTC, TC, SMR, SMT and SMM, and 0.033 $mg{\cdot}kg^{-1}$ for CTC, SDM, and SQX, respectively. So it is sufficiently possible to detect the eight tetracyclines and sulfonamides under their MRLs ($0.1{\sim}0.2mg{\cdot}kg^{-1}$). The average percentage recoveries of sulfonamides and tetracyclines from pig muscle and flatfish spiked standard solution were approximately $80.25{\sim}101.25%$ and $85.77{\sim}121.42%$, respectively. Therefore this method was efficient for simultaneous analysis of eight tetracyclines and sulfonamides.

Keywords

tetracyclines;sulfonamides;pig muscle;flatfish;MSPD;HPLC

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