Acute Oral Toxicity of the Butanol Fraction from Cultured Fruitbody Cordyceps bassiana in Mice

배양 노랑다발동충하초 부탄올분획의 급성경구독성

  • 박은영 (숙명여자대학교 약학대학) ;
  • 박형진 (한림대학교 의과대학) ;
  • 양기숙 (숙명여자대학교 약학대학)
  • Published : 2009.09.30


Cordyceps bassiana is a parasitic fungus and used as a Chinese traditional medicine. It has been called as DongChungHaCho(summer-plant, winter-worm) in China. Acute oral toxicity was examined in male and female ICR mice. Butanol fraction from Cordyceps bassiana(BuCb) was administered orally at a dose of 2,500 mg/kg, 5,000 mg/kg, 10,000 mg/kg. No death and abnormal clinical signs were observed throughout the administration period. The acute toxicity test on mouse did not show any oversign in net body weight gain, food and water consumptions, organ weights, gross pathological findings by different doses of BuCb. Also, biochemical examination revealed no evidence of specific toxicity. These findings show that BuCb has wide margin of safety on acute toxicity with single exposure.


  1. 성재모 (1996) 한국의 동충하초, 28, 교학사, 서울
  2. Li, Z., Li, C., Huang, B and Fan, M (2001) Discovery and demonstration of the telemorph of the Beauveria bassiana(BAls.) Vuill., an important entomogenous fungus. Chinese Science Bulletin 46: 751-753
  3. Lee, J. O., Shrestha, B., Kim, T. W., Sung, G. H. and Sung, J. M. (2007) Stable formation of fruiting body in Cordyceps bassiana. Mycobiology 35: 230-234
  4. Humber, R. A. (2000) Fungal pathogens and parasites of insects. 203-230. In: Priest, F. G. and Goodfellow, M. Eds. Applied Microbial Systematics. Kluwer Acadenic Publishers. Dordrecht
  5. Cho, E. M. and Keyhani, N. O. (2007) EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria bassiana. Mol. Cell. Toxicol. 3: 34.4-34.4
  6. Lee, J. O., Shrestha, B. and Han, S. K (2007) In vitro formation of Cordyceps bassiana fruiting bodies from the Beauveria bassiana Isolate. Int. J. Med. Mush. 9: 323
  7. Sung, J. M., Lee, J. O., and Humber, R. A (2006) Cordyceps bassiana and production of stromata in vitro showing Beauveria anamorph in Korea. Mycobiology. 34: 1-6
  8. 성재모 (1996) 한국의 동충하초, 291, 교학사, 서울
  9. 박찬성, 권충정, 최미애, 박금순, 최경호 (2002) 동충하초의 항산화작용 및 아질산염소거작용. 한국식품저장유통학회지 9: 109-113
  10. 허진철, 남성희, 강석우, 홍인표, 이광길, 박자영, 김경해, 한송이, 이상한 (2007) 동충하초의 균사 및 배양액의 항산 화, 항암, 면역활성의 비교. 한국식품저장유통학회지 14:681-687
  11. Park, S. E., Yoo, H. S. and Jin, C. Y. (2009) Induction of apoptosis and inhibition of telomerase activity in human lung carcinoma cells by the water extract of Cordyceps militaris. Food Chem. Toxicol. 47: 1667-1675
  12. Jin, C. Y., Kim, G. Y. and Choi, Y. H. (2008) Induction of apoptosis by aqueous extract of Cordyceps militaris through activation of caspases and inactivation of Akt in human breast cance cells. J. Microbiol. Biotech.. 18: 1997-2003
  13. Hao, L. (2008) Effectiveness of Cordyceps sinensis alone or in combination with chemotherapy in patients with non-smal cell lung cancer. Biotherapy 22: 345-350
  14. Cheung, J. K., Li, J. and Cheung, A. W. (2009) Cordysinocan, a polysaccharide isolated from cultured Cordyceps, activates immune responses in cultured T-lymphocytes and macrophages. J. Ethnopharmacol. 124: 61-68
  15. Kim, M. N., Oh, S. W., Lee, D. S. and Ham, S. S. (2001) Antioxidantative and antimutagenic effects of the ethanol extract from Cordyceps militaris. Kor. J. POSTHARVEST SCI.. TECHNOL. 8: 109-117
  16. Kim, M. N., Cui, C. B., Lee, D. S. and Ham, S. S. (2001) Cytotoxicity and antigenotxic effects of Cordyceps militaris extracts. J. Kor. Soc. Food Sci. Nutr. 30: 921-927
  17. 허진희 (2002) 노랑다발 동충하초 추출물의 생리활성 탐색에 관한 연구, 강원대 학위논문
  18. Sung, J. M., Kim, T. W., Kim, H. K., Lee, J. O., Yoon, D. H. Kim, G. H., Hwang, J. E. and Park, H. J. (2006) Anti-inflammatory effects of the cultured fruitbody of Cordyceps bassiana butanol fraction. 한국생화학분자생물학회 춘계학술대회집, 390, 서울
  19. 식품의약품안전청 (2005) 의약품등의 독성시험기준 - 식품의약품안전청 고시 제 2005-60호, 서울
  20. 박병권, 임종환, 황윤환, 김명석, 송인배, 이홍기, 한성진, 황미현, 김종우, 이만휘, 박승춘, 윤호인 (2006) 마우스에 대한 Surfactin C의 급성경구독성시험. Toxicol. Res. 22:453-458
  21. 장보윤, 강대길, 이호섭, 김성연 (2008) BDR-29의 랫트에 대한 13주 반복투여 독성에 관한 연구. 생약학회지 39: 60-67
  22. 마진열, 박대훈, 박경수, 도경탁, 신현규 (2007) 쌍화탕 급성독성에 대한 안전성 연구. Kor. J. Orient. Med. 13:161-164
  23. 신지영, 박승춘, 김기현, 배춘식, 김성호, 신동호, 김종춘 (2005) 랫드에서 레몬그라스 정유의 급성독성 평가. Lab. Anim. Res. 21: 122-128
  24. Gosselin, M. E., Belair, G. and Simard, L. (2009) Toxicity of spinosad and Beauveria bassiana to the black cutworm, and the additivity of subletal doses. Biocont. Sci. Tech. 19: 201-217
  25. Dunkel, F. V. and Jaronski, S. T. (2003) Development of a bioassay system for the predator, Xylocoris flavipes (Heteroptera: Anthocoridae), and its use in subchronic toxicity/ Pathogenicity studies of Beauveria bassiana strain. J. Econo. Entomo. 96: 1045-1053
  26. Lee, Y. Y., Wang, Y. H. and Jan, L. C. (2007) Carcinogenicity profile of Beauveria bassiana via genetic and epigenetic toxicities. Plant Protec. Bull. 49: 57-74