Analysis on the distribution of nitrogen and phosphorus removing microorganisms and nitrifying activity in a trickling filter

살수여상에서의 질소, 인 제거 미생물 분포 및 질산화 활성 조사

  • Kim, Dong-Jin (Department of Environmental Sciences & Biotechnology and Institute of Energy & Environment. Hallym University) ;
  • Yoo, Ik-Keun (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Ahn, Dae-Hee (Department of Environmental Engineering and Biotechnology, Myongji University)
  • 김동진 (한림대학교 환경생명공학과 및 에너지.환경연구소) ;
  • 유익근 (울산대학교 화학생명공학부) ;
  • 안대희 (명지대학교 환경생명공학과)
  • Published : 2009.06.30


Trickling filter has been extensively studied for the domestic wastewater treatment especially for the small scale plants in rural area. The performance of the trickling filter depends on the microbial community and their activity in the biofilms on the media. Nitrification. denitrification, and phosphorus removal of the trickling filter from the wastewater depend on the activity and the amount of the specific microorganisms responsible for the metabolism. For the estimation of the performance of a trickling filter, batch nitrification experiment and fluorescence in situ hybridization (FISH) were carried out to measure the microbial activity and its distribution on the media of the trickling filter. Batch nitrification activity measurement showed that the top part of the 1st stage trickling filter had the highest nitrification activity and the maximum activity was 0.002 g $NH_4$-N/g MLVSS${\cdot}$h. It is thought that higher substrate (ammonia) concentration yields more nitrifying bacteria in the biofilms. The dominant ammonia oxidizer and nitrite oxidizer in the biofilm were Nitrosomonas species and genus Nitrospira, respectively, by FISH analysis. Less denitrifiers were found than nitrifiers in the biofilm by the probe Rrp1088 which specifically binds to Rhodobacter, Rhodovulum, Roseobacter, and Paracoccus. Phosphorus accumulating bacteria were mostly found at the surface of the biofilm by probe Rc988 and PAO651 which specifically binds to Rhodocyclus group and their biomass was less than that of nitrifiers.


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