Effects of Chilling and Overwintering Temperature Conditions on the Termination of Egg Diapause of the Ussur Brown Katydid Paratlanticus ussuriensis

갈색여치 알의 휴면타파를 위한 저온처리 및 월동 온도조건의 영향

  • Bang, Hea-Son (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Myung-Hyun (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Jung, Myung-Pyo (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Han, Min-Su (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Na, Young-Eun (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Kee-Kyung (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Deog-Bae (Agro-Ecosystem, Environmental Ecology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kyeong-Yeoll (Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • 방혜선 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 김명현 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 정명표 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 한민수 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 나영은 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 강기경 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 이덕배 (농촌진흥청 국립농업과학원 농업환경부 기후변화생태과) ;
  • 이경열 (경북대학교 농업생명과학대학 응용생명과학부)
  • Published : 2009.06.30


Temperature effects on diapause termination of Paratlanticus ussuriensis eggs were studied by measuring embryonic development and hatching rates at various conditions of indoor chilling and overwintering temperatures. Diapausing eggs of P. ussuriensis did not hatch at continued incubation at $25^{\circ}C$ and even after chilling for once at either $5^{\circ}C$ or $10^{\circ}C$ for 30, 45 and 60 days. In addition, double chillings at $5^{\circ}C$ with a 90 days interval at $25^{\circ}C$ did not induce hatching of diapausing eggs. However, double chillings at $10^{\circ}C$ induced hatching at 3.6${\sim}$26.7%. When eggs were incubated at $25^{\circ}C$ after chilling for once at $5^{\circ}C$ for various periods, those weights were not changed but those chilled at $10^{\circ}C$ gradually increased to approximately 1.5 times. When 60-days-old eggs were artificially deposited under the soil at three different mountain sites in September 2007, the hatching rates of the first-overwintered eggs were 11.3, 3.5 and 4.1% and those of the second-overwintered eggs were 25.1, 21.6 and 0.4% at Hoepori, Bitanri and Hwasanri, respectively. Most eggs were hatched from mid-March to mid-April but little bit earlier in southern regions. During the hatching period soil temperatures in three tested locations were around 8 to $12^{\circ}C$. In overall, diapausing eggs of P. ussuriensis were greatly influenced by chilling temperature conditions and those repeated cycles, and may required overwintering for one or two times to hatch for the post-embryonic development.


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