Phosphate Solubilization and Plant Growth Promotion by Crop Associated Bacteria

인산용해미생물에 의한 불용성 인의 용해와 식물생장에 미치는 영향

  • Na, Jung-Heang (Division of Applied BioScience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Choi, Jin-Ho (National Institute of Horticultural & Herbal science, RDA) ;
  • Jin, Rong-De (Division of Applied BioScience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Ko, Hyun-Sun (Division of Applied BioScience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Ro-Dong (Division of Applied BioScience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kim, Kil-Yong (Division of Applied BioScience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
  • 나정행 (전남대학교 응용생물공학부) ;
  • 최진호 (농촌진흥청 국립원예특작과학원) ;
  • 김영덕 (전남대학교 응용생물공학부) ;
  • 고현선 (전남대학교 응용생물공학부) ;
  • 박노동 (전남대학교 응용생물공학부) ;
  • 김길용 (전남대학교 응용생물공학부)
  • Received : 2008.09.22
  • Accepted : 2008.11.20
  • Published : 2009.02.28

Abstract

Fourteen bacterial strains were isolated from crop rhizosphere and identified as phosphate solubilizing bacteria (PSB) by 16S rRNA analysis. Only 3 strains exhibited a strong ability to solubilize insoluble phosphate in agar medium containing a hydroxyapatite. The rates of P solubilization by isolates were ranged from 200 and $2300\;mg\;L^{-1}$, which are inversely correlated with pH in culture medium. Furthermore, HPLC analyses reveal the production of organic acid from the culture filtrates of PSB. Among these, strain Acinetobacter sp. released only gluconic acid, Pseudomonas orientalis produced gluconic acid which was subsequently converted into 2-ketogluconic acid, and Enterobacter asburiae released acetic acid and succinic acid. On the other hand, P. orientalis and E. asburiae released $372\;mg\;L^{-1}$ and $191\;mg\;L^{-1}$ of IAA into broth culture, respectively, while Acinetobacter sp. did not produce IAA. Furthermore, in vivo study showed that plant growth promoting effect by bacteria generally seemed to be increased IAA production and phosphate solubilization.

Keywords

Phosphate solubilizing bacteria (PSB);Cucumber;IAA;Plant growth promoting rhizobacteria(PGPR)

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