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Evaluation of Probiotics as an Alternative to Antibiotic on Growth Performance, Nutrient Digestibility, Occurrence of Diarrhea and Immune Response in Weaning Pigs

항생제 대체제로서 생균제가 이유자돈의 성장능력 및 영양소 이용률, 설사 빈도, 면역 반응에 미치는 영향

  • Jang, Young-Dal (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Oh, Hee-Kyung (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Piao, Long-Guo (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hyun-Bong (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Yun, Jin-Hyeon (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Yoo-Yong (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 장영달 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 오희경 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 박용국 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 최현봉 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 윤진현 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 김유용 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원)
  • Received : 2008.12.24
  • Accepted : 2009.02.19
  • Published : 2009.02.01

Abstract

This study was conducted to determine the effects of probiotics supplementation on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. Treatments were 1) NC (basal diet), 2) PC (basal diet + 0.12% avilamycin) and 3) A (basal diet + 0.2% Aspergillus oryzae), 4) B (basal diet + 0.2% Lactobacillus casei), 5) C (basal diet + 0.2% Bacillus subtilis), 6) D (basal diet + 0.2% Lactobacillus crispatus). A total of 120 pigs ($7.16\pm0.01$ kg average body weight, weaned at $23\pm3$days of age) were assigned to 6 treatments, 5 replicates and 4 pigs per pen in a randomized complete block (RCB) design. During the whole experimental period, body weight (P<0.01), average daily gain (ADG; P<0.01), and average daily feed intake (ADFI; P<0.05) of treatment PC were higher than other treatments. However, the probiotics treatments tended to increase ADG and G:F ratio compared to treatment NC. The G:F ratio in treatment A (Aspergillus oryzae) was similar to treatment PC during the whole experimental period (P<0.05). The supplementation of probiotics in the diet of weaning pig did not change nutrient digestibility (dry matter, crude protein, crude fat and ash) and nitrogen retention of weaning pigs. In blood urea nitrogen (BUN) concentration, treatment B had lower value than other treatments at 2 and 4 weeks (P<0.05). Treatments PC and C tended to decrease diarrhea score than other treatments (P=0.18). At 3h after lipopolysaccharide (LPS) injection, treatments NC and PC had higher count of $CD^{4+}$ T-cells than probiotics treatments, and treatment C showed the lowest value (P<0.01). There were no differences on count of $CD^{8+}$ T-cells and $CD^{4+}:CD^{8+}$ ratio among all treatments (P>0.10). These results suggest that the dietary probiotics are likely able to improve the growth performance, occurrence of diarrhea and immune response although they do not have similar effects like antibiotics in weaning pigs.

Acknowledgement

Supported by : 농림수산식품부

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