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Linkage Disequilibrium and Gene Expression Analyses of IGF2 Gene in Korean Native Pigs

재래돼지를 이용한 IGF2 유전자의 연관불균형과 유전자발현양상에 대한 분석

  • Li, Song-Lan (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Li, Xiaoping (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Choi, Bong-Hwan (Animal Genomics and Bioinformatics Division, National Livestock Research Institute, RDA) ;
  • Lee, Cheol-Koo (Collelge of Life Sciences & Biotechnology, Korea University) ;
  • Cho, Byung-Wook (Department of Animal Science, Pusan National University) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Kim, Kwan-Suk (College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 이송란 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 이소평 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 최봉환 (농촌진흥청 농촌진흥청 국립축산과학원) ;
  • 이철구 (고려대학교 생명과학대학 생명공학부) ;
  • 조병욱 (부산대학교 생명자원과학부) ;
  • 김종주 (영남대학교 생명공학부) ;
  • 김관석 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과)
  • Received : 2008.10.14
  • Accepted : 2009.02.11
  • Published : 2009.02.01

Abstract

Insulin-like growth factor 2 (IGF2) is the first identified imprinted gene, which is paternally expressed in multiple mammalian species. A paternally expressed QTL for muscle growth and backfat thickness (BFT) has previously been identified near the IGF2 locus on the distal tip of pig chromosome 2 (SSC2p). Therefore the IGF2 gene is considered an economically important candidate gene for pig industry. Herein, this study explored genetic variation of IGF2 for in3-G3072A, in7-G162C and a new SNP in intron7 (C1589T) in Korean native pig (KNP) and commercial pig breeds, and detected their linkage disequilibrium within these breeds. Furthermore we investigated the effect of in3-G3072A on IGF2 gene expression in post-natal muscle and backfat tissues. The real-time quantitative PCR results showed that animals inherited allele G from a KNP sire had significant higher IGF2 gene expression in backfat tissue than those inherited allele A from a Yorkshire sire, however opposite situation in muscle. These results demonstrated the allele 3072G is associated with a higher IGF2 gene expression in fat tissues, but low gene expression in muscle tissues when compared with the 3072A allele. These results suggest that KNP with lower muscle mass and higher fat deposition might be associated with a higher frequency of the 3072G allele, and selecting KNP based on IGF2 genotypes could result in an economic benefit to KNP producers.

Acknowledgement

Grant : DNA 마커 도움선발을 이용한 한국재래돼지 개량과 상업적 비육흑돼지 계통조성

Supported by : 농촌진흥청

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