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General Properties of Phytase Produced by Fluorescent Pseudomonas sp. BUN1

토양세균 Fluorescent Pseudomonas sp. BUN 1 균주 유래의 파이테이즈(Phytase)의 일반적 특성규명

  • Cho, Jaie-Soon (Department of Animal Sciences & Environment, College of Animal Bioscience & Technology, Konkuk University)
  • 조재순 (건국대학교 동물생명과학대학 동물생산환경학)
  • Received : 2009.01.08
  • Accepted : 2009.04.01
  • Published : 2009.04.01

Abstract

A bacterial strain producing intracellular phytase was isolated from cultivable soil near cowsheds and identified as a fluorescent Pseudomonas sp. BUN1. The BUN1 phytase, partially purified by cation and anion exchange chromatography, exhibited its optimal activity at $40^{\circ}C$ and pH 5.5. As for substrate specificity, it was very specific for phytate and showed little activity on other phosphorylated conjugates. Its activity was greatly inhibited by metal ions such as $Cu^{2+}$, $Cd^{2+}$, and $Zn^{2+}$. Addition of corn starch to PSM (phytasesynthetic medium) [0.5% sodium phytate, 0.5% $(NH_4)_2SO_4$, 0.5% KCl, 0.01% $MgSO_4\cdot7H_2O$, 0.01% $CaCl_2\cdot2H_2O$, 0.01% NaCl, 0.001% $FeSO_4\cdot7H_2O$, 0.001% $MnSO_4\cdot4H_2O$; pH 6.5] for the phytase production significantly induced its enzyme activity in comparison with other carbon sources tested.

Keywords

Bacterial;Intracellular;Pseudomonas;Phytase;Phytate

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