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Commercial Application of Porcine MC1R Gene Polymorphisms to Korean Pork Industry

돼지 MC1R 유전자변이의 양돈산업 적용

  • Ha, You-Kyoung (Department of Animal Science, Chungbuk National University) ;
  • Choi, Jung-Suk (Department of Animal Science, Chungbuk National University) ;
  • Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Choi, Yang-Il (Department of Animal Science, Chungbuk National University) ;
  • Lee, Seug-Soo (National Livestock Research Institute) ;
  • Choi, Jae-Won (ChungBuk Institute of Livestock and Veterinary Research) ;
  • Jeon, Soon-Hong (ChungBuk Institute of Livestock and Veterinary Research) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
  • 하유경 (충북대학교 농업생명환경대학 축산학과) ;
  • 최정석 (충북대학교 농업생명환경대학 축산학과) ;
  • 김상욱 (충북대학교 농업생명환경대학 축산학과) ;
  • 최양일 (충북대학교 농업생명환경대학 축산학과) ;
  • 이승수 (국립축산과학원 개량평과과) ;
  • 최재원 (충청북도 축산위생연구소) ;
  • 전순홍 (충청북도 축산위생연구소) ;
  • 김관석 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2009.05.08
  • Accepted : 2009.06.09
  • Published : 2009.06.01

Abstract

The pork from black-coated pigs is famous among-consumers for better eating quality. The loci affecting black coat color was identified in pig chromosome 6 in which several genetic effects on pork quality have been reported. The melanocortin 1 receptor (MC1R) gene is a major gene which plays a key role in regulation of eumelanin (black/brown) and phaeomelanin (red/yellow). In this study, the MC1R gene polymorphism was analyzed for pig breed determination and genetic association with pork quality traits. MC1R Ala243Thr variation was analyzed to determine a specific genotype for four commercial pig breeds (Landrace, Yorkshire, Berkshire and, Duroc) and a Korean native pigs (KNP). Then we developed original KNP-specific DNA markers to determine the pork from black-coated pigs using MC1R DNA sequences. The total length of the MC1R coding sequence ranged 1451bp in KNP. KNP had the 0201 allele pertaining to $E^{D1}$ but some of the KNP had the $E^P$ allele, probably reflecting the geneticintrogression of $E^P$ allele into KNP. Furthermore, a relationship between Leu102Pro single nucleotide polymorphism (SNP) genotype and pork quality phenotype were analyzed in F2 reciprocal-crossbred population between KNP and Yorkshire. Association analysis indicated that the allele of the MC1R gene has no effect on pork quality. These results suggest that black coat-color is not directly associated with preferred pork quality, but the black-coat color pig breed may have other genetic components for superior pork quality.

Keywords

MC1R;KNP;Polymorphism;Pork quality

Acknowledgement

Grant : DNA 마커 도움선발을 이용한 한국재래돼지 개량과 상업적 비육흑돼지 계통조성

Supported by : 충북대학교

References

  1. Cho, I. C., Lee, J. K., Jung, J. K., Yang, B. S., Kang, S. Y. and Kim, B. W. 2002. Studies on the MC1R Gene Frequencies in Landrace, Large White, Duroc and Jeju Native Black Pigs. J. Anim. Sci. & Technol. (Kor). 44, 207-212. https://doi.org/10.5187/JAST.2002.44.2.207
  2. Cho, I. C., Jung, Y. H., Jung, J. K., Seong, P. N., Oh, W. Y., Ko, M. S., Kim, B. W., Lee, J. G. and Jeon, J. T. 2004. Detection of Novel Genetic Variations of the MC1R*3 Allele in Pig (Sus scrofa). J. Anim. Sci. & Technol. (Kor). 46, 1-6. https://doi.org/10.5187/JAST.2004.46.1.001
  3. Dun, G., Li, X., Cao, H., Zhou, R. and Li, L. 2007. Variation of Melanocortin Receptor 1 (MC1R) Gene in Three pig Breeds. J. Genet Genomics. 34, 777-782. https://doi.org/10.1016/S1673-8527(07)60088-5
  4. Fang, M., Larson, G., Ribeeiro, H. S., Li, N. and Andersson, L. 2009. Contrasting mode of Evolution at a Coat Color Locus in Wild and Domestic Pigs. PLoS Genetics. 5, e1000341. https://doi.org/10.1371/journal.pgen.1000341
  5. Fortin, A., Robertson, W. M. and Tong, A. K. W. 2005. The eating quality of Canadian pork and its relationship with intramuscular fat. Meat Sci. 69, 297-305. https://doi.org/10.1016/j.meatsci.2004.07.011
  6. Kerong, S., Aiguo, W., Ning, L. and Xuemei, D. 2004. Single nucleotide polymorphism analysis in melanocortin receptor (MC1R) of Chinese native pig. Sci China C Life Sci. 47, 287-292.
  7. Kijas, J. H., Wales, R., Tornsten, A., Chardon, P., Moller, M., and Andersson, L. 1998. Melanocortin Receptor 1 (MC1R) Mutations and Coat color in Pigs. Genetics. 150, 1177-1185.
  8. Kim, T. H., Yoon, D. H., Nho, W. G., Yeon, S. H., Kim, N. S., Lee, H. J., Cheong, I. C. and Han, J. Y. 2001. Genetic Variation of Melanocortin Receptor 1 (MC1R) in Korean Native Pig. J. Anim. Sci. & Technol. (Kor). 43, 293-302.
  9. Klungland, H., Vage. D. I., Gomez, R. L., Adalsteinsson, S. and Lien, S. 1995. The role of Melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm Genome. 6, 636-639. https://doi.org/10.1007/BF00352371
  10. Lee. S. S., Yang, B. S., Jung, J. K., Ko, S. B., Oh, S. J., Yang, Y. H., Kim, K. I., Li, Z. D. and Feng, S. T. 2001. Comparison of the Genotype of melanocortin Receptor 1 (MC1R) Gene in Korean-native and Chinese-native Pigs. J. Anim. Sci. & Technol. (Kor). 43, 1-8.
  11. Mariani, P., Johansson Moller, M., Hoyheim, B., Marklund, L. and Davies, W. 1996. The extention coat color locus and the loci for Blood Group O and Tyrosine Aminotransferase are on pig chromosome 6. J. Hered. 87, 272-276. https://doi.org/10.1093/oxfordjournals.jhered.a023000
  12. Marklund. L., Johansson, M., Sandberg, K. and Andersson, L. 1996. A missense mutation in the gene for melanocytestimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses. Mamm. Genome. 7, 895-899. https://doi.org/10.1007/s003359900264
  13. Newton, J. M., Wilkie, A. L., He, L., Jordan, S. A. and Metallinos, D. L. 2000. Melanocortin 1 receptor variation in the domestic dog. Mamm. Genome. 11, 24-30. https://doi.org/10.1007/s003350010005
  14. Takeuchi, S. H., Suzuki, H., Yabuuchi, M. and Takahashi, S. 1996. A possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chiken. Biochm. Biophys. Acta. 1308, 164-168. https://doi.org/10.1016/0167-4781(96)00100-5
  15. Valverde, P., Healy, E., Jackson, I., Rees, J. L. and Thody, A. J. 1995. Variants of the melanocyte-stimulating hormone receptor gene are associated with red hair and fair skin in humans. Nature Genet. 11, 328-330. https://doi.org/10.1038/ng1195-328
  16. van der Wal, P. G., Engelb, B. and Hulsegge, B. 1997. Causes for variation in pork quality. Meat Sci. 46, 319-327. https://doi.org/10.1016/S0309-1740(97)00026-0