Characterization of the Bovine FASN Gene Variation for Carcass and Beef Quality Traits in Hanwoo

소 FASN 유전자 변이의 연관불균형과 한우 도체형질에 미치는 영향

  • Li, Song-Lan (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Sang-Wook (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Lee, Jung-Jae (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Lee, Jun-Heon (College of Agriculture, and Life Sciences, Chungnam National University) ;
  • Yoon, Du-Hak (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Jeong, Young-Chul (Jung Pork & Customer Institute) ;
  • Jeon, Soon-Hong (Institute of Livestock and Veterinary Research) ;
  • Choi, Jae-Won (Institute of Livestock and Veterinary Research) ;
  • Kim, Nae-Su (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Kwan-Suk (College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 이송란 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 김상욱 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 이중재 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 이준헌 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 윤두학 (농촌진흥청 국립축산과학원) ;
  • 김종주 (영남대학교 생명공학부) ;
  • 정영철 (정피엔씨연구소) ;
  • 전순홍 (충청북도 축산위생연구소) ;
  • 최재원 (충청북도 축산위생연구소) ;
  • 김내수 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과) ;
  • 김관석 (충북대학교 농업생명환경대학 응용생명환경학부 축산학과)
  • Received : 2009.04.24
  • Accepted : 2009.06.08
  • Published : 2009.06.01


Fatty acid synthase (FASN) is a multi-functional enzyme with a central role in the synthesis of long-chain fatty acid and has been considered as a positional candidate gene for BTA 19 quantitative trait loci (QTL) affecting milk-fat content and fatty acid composition. In this study, we sequenced the FASN gene in several cattle breeds including Hanwoo and imported beef cattle, and identified novel DNA polymorphisms and their linkage relationship in Hanwoo. We found a significant frequency difference of the FASN (AF285607) g.17924 A$\rightarrow$G polymorphism between Hanwoo (70%) and other breeds and this polymorphism has been known for an association with fatty acid composition in Angus. Furthermore, by direct DNA sequencing in 18 unrelated Hanwoo, we identified 27 SNPs including nine novel variations in the FASN gene. Among 27 SNPs identified in the FASN gene, four SNPs were further genotyped in 100 Hanwoo and 96 imported beef cattle, and analyzed for haplotype construction and association with beef quality traits. We performed haplotype block and linkage disequilibrium studies using four selected SNPs. Two different haplotype blocks (block A: g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$ A; block B: g.13125 C$\rightarrow$T and g.17924 G$\rightarrow$A) were constructed and the block A in particular had a very high r2 (0.936), which indicated a nearly complete linkage disequilibrium existed between the g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$A polymorphisms. A total of four major haplotypes (frequency > 0.05) were identified with the four polymorphisms including TATG (0.36), CGCG (0.31), CGTA (0.19) and TACG (0.06). Statistical association analysis revealed that the g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$A polymorphisms in the FASN were significantly associated with meat color (P=0.004) and texture (P=0.0114). The g.13125 C$\rightarrow$T and g.17924 G$\rightarrow$A polymorphisms in the FASN were also significantly associated with back-fat thickness and quantity index (P=0.0179 and 0.0495, respectively). Our findings suggested that the FASN gene polymorphisms may be used for determining the (unsaturated) fatty acid contents and carcass trait in the Hanwoo beef.


FASN;SNPs;HRM assay;Hanwoo;Beef quality


Grant : 한우 육량.육질 조기 선발용 DNA Kit 산업화 기술 개발

Supported by : 농림수산식품부


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