Effect of Immunoglobulin Y on Growth Performance and Blood Immunological Parameters in Holstein Calves

난황항체(IgY) 급여가 Holstein 송아지의 성장능력 및 면역관련 혈액 지표에 미치는 영향

  • Jung, Hee-Sung (Dept. of Animal Science & Biotechnology, Jinju National University) ;
  • Jung, Keun-Ki (Moksan Hanwoo Research Institute) ;
  • Jang, In-Surk (Dept. of Animal Science & Biotechnology, Jinju National University)
  • 정희승 (진주산업대학교 동물생명과학과) ;
  • 정근기 (동물생명산업센터, 목산한우연구소(사)) ;
  • 장인석 (진주산업대학교 동물생명과학과)
  • Received : 2009.07.16
  • Accepted : 2009.08.16
  • Published : 2009.08.01


A total of fourteen, 1-wk-old male Holstein calves were allotted into two groups consisted of control (CON) and IGY which was orally administrated with immunoglobulin yolk (IgY) for 1wk. Calves in both groups were provided with milk replacer according to feeding program and had ad libitum access to timothy hay for the entire experimental period (7wks). At 0, 7 and 49 day of experiment, blood samples were collected from the jugular vein of calves to investigate blood biochemical profiles and the differential count (%) of white blood cell (WBC). We also monitored growth performance and colony forming unit (CFU) of fecal microbial population in calves. The adminstration of IgY in calves did not affect body weight and weight gain during 49 days feeding trial compared with control group. The CFU of E. coli and Lactobacilli in the feces of calves were not significantly affected by IgY treatment, whereas the score of the calf scours during day 43 to 49 in IgY group showed a significant (P<0.05) solid type. There were no differences in plasma biochemical components including total protein, albumin, immunoglobulin and the other indicators. As for WBC differential count (%), there was no statistical difference in the percentages of neutrophil, lymphocyte, monocyte, eosinophil and basophil at 0, 7 and 49 days after the oral supplementation of IgY. In conclusion, the oral supplementation of IgY as an immunostimulant did not affect growth performance, fecal microbial population, blood biochemical profile and WBC differential count in Holstein calves.


Calves;IgY;WBC differential count;CFU


  1. Arthington, J. D., Cattell, M. B., Quigley, J. D., McCoy, G. C. and Hurley, W. L. 2000. Passive IgG transfer in newborn calves fed colostrum or spray-dried serum protein alone or as a supplement to colostrum of varying quality. J. Dairy Sci. 83(12):2834-2838.
  2. Baron, J. M., Heise, R., Blaner, W. S., Neis, M., Joussen, S., Dreuw, A., Marquardt, Y., Saurat, J. H., Merk, H. F., Bickers, D. R. and Jugert, F. K. 2005. Retinoic acid and its 4-oxo metabolites are functionally active in human skin cells in vitro. J. Invest. Dermatol. 125(1):143-153.
  3. Brown, G. B., Bolin, S. R., Frank, D. E. and Roth, J. A. 1991. Defective function of leukocytes from cattle persistently infected with bovine viral diarrhea virus, and the influence of recombinant cytokines. Am. J. Vet. Res. 52:381.
  4. Erhard, M. H., Gobel, E., Lewan, B., Losch, U. and Stangassinger, M. 1997. Systemic availability of bovine IgG and chicken IgY after feeding colostrum and whole egg powder to newborn calves. Arch. Tierernahr. 50:369-380.
  5. Garry, F. B., Adams, R., Cattell, M. B. and Dinsmore, R. P. 1996. Comparison of passive IgG transfer to dairy calves fed colostrum or commercially available colostral-supplement products. J. Am. Vet. Med. Assoc. 208(1):107-110
  6. Girard, F., Batisson, I., Martinez, G., Breton, C., Harel, J. and Fairbrother, J. M. 2006. Use of virulence factor-specific egg yolk-derived IgGs as a promising alternative to antibiotics for prevention of attaching and effacing E. coli infections. FEMS Immunol. Med. Microbiol. 46(3):340-350.
  7. Godden, S. M., Smith, S., Feirtag, J. M., Green, L. R., Wells, S. J. and Fetrow, J. P. 2003. Effect of on-farm commercial batch pasteurization of colostrum on colostrum and serum IgG concentrations in dairy calves. J. Dairy Sci. 86(4):1503-1512.
  8. Hoerlein, A. and Marsh, C. L. 1957. Studieson the epizootiology of shipping fever in calves. J. Am. Vet. Med. Assoc. 131:123.
  9. Ikemori, Y., Kuroki, M., Peralta, R. C., Yokoyama, H. and Kodama, Y. 1992. Protection of neonatal calves against fatal enteric colibacillosis by administration of chicken egg yolk powder from hens immunized with K99-pilated enterotoxigenic E. coli. Am. J. Vet. Res. 53:2005-2008.
  10. Kim, D., Lyoo, Y. S., Lyoo H. S. and Yoon C. K. 1990. Etiology and clinical aspects of diarrhea of Korean native calves during the suckling periods. Kor. J. Vet. Res. 30(2): 255-260.
  11. Kim, J. M., Woo, S. R., Kwon, C. H., Kim, J. Y. and Huh, W. 1998. Development of preventive method for enterotoxigenic colibacillosis using egg yolk antibodies against colibacillosis of piglets. Korean J. Vet. Res. 34:837-842.
  12. Logan, E. F., Pearson, G. R. and McNully, M. S. 1977. Studies on the immunity of the calf to colibacillosis-VII: The experimental reproduction of enteric colibacillosis in colostrumfed calves. Veterinary Record. 101:443-446.
  13. Menge, C., Neufeld, B., Hirt, W., Schmeer, N., Bauerfeind, R., Baljer, G. and Wieler, L. H. 1998. Compensation of preliminary blood phagocyte immaturity in the newborn calf. Vet Immunol. Immunopathol. 62(4):309-321.
  14. Oh, T. H. and Han, H. R. 1996. Protective effect of chicken egg yolk antibody in colostrums-deprived neonatal puppies. Korean J. Vet. Res. 36: 903-913.
  15. Osame, S., Ichojje, S., Ohata, C., Watanabe, W. and Goto, H. 1991. Efficacy of colostrial immunoglobulins for therapeutic and preventive treatments of calf diarrhea. J. Vet. Med. Sci. 53(1):87-91.
  16. Quigley, J. D. III Fike D. L., Egerton, M. N., Drewry, J. J. and Arthington, J. D. 1998. Effects of a colostrum replacement product derived from serum on immunoglobulin G absorption by calves. J. Dairy Sci. 81(7):1936-1939.
  17. Quigley, J. D. III and Welborn, M. G. 1996. Influence of injectable immunoglobulin on serum immunoglobulin concentrations in dairy calves. J. Dairy Sci. 79(11):2032-2037.
  18. SAS. 1996. User’s Guide: Statistics Version 6.12 Edition. SAS inst, Inc., Cary., NC.
  19. Yokoyama, H., Paralta, R. C., Diaz, R., Sendo, S., Ikemori, Y. and Kodama, Y. 1992. Passive protective effect of chicken egg yolk immnunoglobulins against experimental enterotoxigenic E. coli infection in neonatal piglets. Infect. Immuno. 60: 998-1007.
  20. 김 두, 유영수, 유한상, 윤충근. 1990. 한우송아지의 포유기간 중의 설사발생에 관한 연구. Korean J. Vet. Res., 30(2): 250-260.
  21. 백순용. 1990. 백순용의 소 질병관리. 종합출판문화사.
  22. 정근기, 강수원, 김경훈, 김내수, 서국현, 성환우, 송만강, 오 영균, 원유석, 윤종택, 이문연, 최창본. 2005. 한우. 전국한우 연구회. 대원문화인쇄. 서울.
  23. 서정원. 2006. 건강한 송아지와 설사송아지에서 혈중 retinol 과 $\alpha$-tocopherol의 비교. 제주대학교 대학원. 석사논문.
  24. 양일석, 강창원, 김상근, 김주헌, 김태완, 나승열, 박수현, 박 전홍, 윤영원, 이국경, 이상목, 이만휘, 이장헌, 한호재. 2004. 수의생리학. 광일문화사. 서울
  25. 內藤善久, 김덕환, 신형태. 1993. 가축설사의 진단: 송아지 설사에 대하여. Kor. J. Vet. Clin. Med. 1-7.