- Volume 51 Issue 4
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Effects of Dietary Garlic Powder on Growth Performance and mRNA Expression of Hepatic HMG-CoA Reductase in Broiler Chickens
육계 사료 내 마늘분말의 첨가급여가 육계 성장과 HMG-CoA Reductase의 mRNA 발현에 미치는 영향
- You, Sun-Jong (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University) ;
- Ahn, Byoung-Ki (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University) ;
- Kang, Chang-Won (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University)
- Received : 2009.02.20
- Accepted : 2009.07.14
- Published : 2009.08.01
This experiment was conducted to investigate the effects of dietary garlic powder (GP) on growth performance and mRNA expression of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) in broiler chickens. Day-old male chicks (Ross, n=270) were fed diets containing 0% [positive control (PC) with antibiotics or negative control (NC) without antibiotics], 1%, 3% and 5% GP for 6 wks. There were no significant differences in feed intake among the treatments throughout the experimental period. Body weight gains in groups fed dietary GP at 3% and 5% were significantly higher than that of NC group (P<0.05). Feeding GP up to 5% did not exert any adverse effect on weight gain and feed intake. There were no significant differences in the relative weights of liver, spleen, cecum and breast muscle. The content of meat cholesterol in GP containing dietary groups tended to be reduced as compared to NC group. Average infectious bronchitis antibody titers in chicks fed GP containing diets were significantly higher than that of NC group (P<0.05). The mRNA expression of hepatic HMG-CoA reductase was reduced by dietary GP. These results indicate that dietary GP has growth promoting effects and tended to alter cholesterol metabolism in broiler chickens.
Garlic;HMG-CoA reductase;Growth performance;Broiler chicken
Supported by : 농림수산식품부
- Adams, M. L., Sullivan, D. M., Smith, R. L. and Richter, E. F. 1986. Evaluation of direct saponification method for determination of cholesterol in meats. J. Assoc. Off. Anal. Chem. 69(5):844-846.
- Bast, A., Chandler, R. F., Choy, P. C., Luc, L. M., Delulle, M., Gruenwald, J., Bart, S., Halkes, A., Keller, K., Koeman, J. H., Peters, P., Przyrembel, H., de Ree, E. M., Renwick, A. G. and Vermeer, I. T. M. 2002. Botanical health products; positioning and requirements for effective and safe use. Environ. Toxicol. & Pharm. 12:195-211. https://doi.org/10.1016/S1382-6689(02)00035-2
- Campbell, J. H., Efendy, J. L., Smith, N. J. and Campbell, G. R. 2001. Molercular basis by which garlic suppresses atherosclerosis. J. Nutr. 131:1006-1009.
- Cheng, M. H. and Jung, T. C. 1981. Effect of allithiamine on sacroma-180 tumor growth in mice. Tai-wan I Husuch Hui Tsa chif 80(4):385-393.
- Chomczylnski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
- Chowdhury, S. R., Chowdhury, S. D. and Smith, T. K. 2002. Effects of dietary garlic on cholesterol metabolism in laying hens. Poultry Sci. 81:1856-1862.
- Duncan, D. B. 1955. Multiple range and multiple F test. Biometr. 11:1-42. https://doi.org/10.2307/3001478
- Elkin, R. G., Freed, M. B., Kieft, K. A. and Newton, R. S. 1993. Alteration of egg yolk cholesterol content and plasma lipoprotein profiles following administration of a totally synthetic HMG-CoA reductase inhibitor to laying hens. J. Agric. Food Chem. 41:1094-1101. https://doi.org/10.1021/jf00031a016
- Lau, B. H. S. 2001. Suppression of LDL oxidation by garlic. J. Nutr. 131:985-988.
- Elkin, R. G., Zhong, Z. Y., Donkin, S. S., Buhman, K. K., Story, J. A., Turek, J. J., Porter Jr, R. E., Anderson, M., Homan, R. and Newton, R. S. 1999. Select 3-Hydroxy-3methylglytaryl-coenzyme A reductase inhibitors vary in their ability to reduce egg yolk cholesterol levels in laying hens through alteration of hepatic cholesterol biosynthesis and plasma VLDL composition. J. Nutr. 129:1010-1019.
- Fleischauer, A. T. and Arab, L. 2001. Garlic and cancer: A critical review of the epidemiologic literature. J. Nutr. 131: 1032-1040.
- Folch, G., Lees, M. and Stanley, G. H. S. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
- Gebhardt, R. 1993. Multiple inhibitory effects of garlic extracts on cholesterol biosynthesis in hepatocytes. Lipids 24(7):613-619.
- Gebhardt, R. and Beck, H. 1996. Differential inhibitory effects of garlic derived organosulfur compound on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids 31(12): 1269-1276. https://doi.org/10.1007/BF02587912
- Konjufca, V. H., Pesti, G. M. and Bakalli, R. I. 1997. Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poultry Sci. 76:1264-1271.
- Kyo, E., Uda, N., Kasuga, S. and Itakura, Y. 2001. Immunomodulatory effects of aged garlic extract. J. Nutr 131: 1075-1079.
- Lamm, D. L. and Riggs, D. R. 2001. Enhanced immunocompetence by garlic: Role in bladder cancer and other malignancies. J. Nutr. 131:1067-1070.
- Liu, L. and Yeh, Y. Y. 2000. Inhibition of cholesterol biosynthesis by organosulfur compounds derived from garlic. Lipids 35(2):197-203. https://doi.org/10.1007/BF02664770
- Milner, J. A. 2001. A historical perspective on garlic and cancer. J. Nutr. 131:1027-1031.
- Qureshi, A. A., Abuirmeileh, N., Din, Z. Z., Elson, C. and Burger, W. C. 1983a. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fraction of garlic. Lipids 18:343-348. https://doi.org/10.1007/BF02537229
- Qureshi, A. A., Din, Z. Z., Abuirmeileh, N., Burger, W. C., Ahmad, Y. and Elson, C. E. 1983b. Suppression of avian hepatic lipid metabolism by solvent extract of garlic: Impact on serum lipids. J. Nutr. 113:1746-1755.
- Rahman, K. 2001. Historical perspective on garlic and cardiovascular disease. J. Nutr. 131:977-979.
- SAS Institute Inc. 1989. SAS/STAT User's Guide. Version 6, 4th Edition. SAS Institute Inc., Cary, NC.
- Schaefer, E. J. 2002. Lipoproteins, nutrition, and heart disease. Am. J. Clin. Nutr. 75:191-212.
- Sharma, K. K. and Sharma, S. P. 1979. Effect of onion and garlic on serum cholesterol on normal subjects. Mediscope. 22(7):134-136.
- Slowing K., Gando, P., Sanz, M., Ruiz, E. and Tejerina, T. 2001. Study of garlic extracts and fractions on cholesterol plasma levels and vascular reactivity in cholesterol-fed rats. J. Nutr. 131:994-999.
- Tompson, R. H. and Merola, G. V. 1993. A simplified alternative to the AOAC official method for cholesterol in multicomponent foods. J. AOAC inter. 76(5):1057-1068.
- Yeh, Y. Y. and Liu, L. 2001. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: Human and animal studies. J. Nutr. 131:989-993.
- 문영자, 염금희, 성창근. 2002. HMG-CoA reductase 저해제 탐색과 가금의 콜레스테롤 저하 효과. 한국식품영양학회. 15 (4):307-313.
- 윤병선, 남기택, 김창원, 강창원, S. Ohtani, K, Tanaka. 1996. 육계 사료 내 마늘의 첨가가 육계의 생산성과 HMG-CoA reductase에 미치는 영향. 한국가금학회지. 23(3):129-134.
- 윤병선, 채현석, 김석철, 김동운, 안종남, 김용곤. 1998. 산란 계에 대한 마늘의 급여 효과. 한국영양사료학회지. 22(6):357-362.
- 최용순, 이상영. 1992. 혈청콜레스테롤과 3-hydroxy-3-methylglutaryl coenzyme a reductase. 한국영양식량학회지. 21(5): 580-593.
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