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Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite loci in Domestic Beef Cattle

초위성체 DNA표지인자를 이용한 국내 육우집단의 품종특성 및 개체식별 체계설정

  • Kim, Sang-Wook (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Jang, Hee-Kyung (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Kwan-Suk (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Yoon, Du-Hak (National Institute of Animal Science, RDA) ;
  • Kang, Seong-Ho (Department of Chemistry and Research Institute of Physics and Chemistry, Chonbuk National University) ;
  • Jung, Hyo-Il (Department of Mechanical Engineering, Yonsei University) ;
  • Cheong, Il-Cheong (Hankyong National University)
  • 김상욱 (충북대학교 농업생명환경대학 축산학과) ;
  • 장희경 (충북대학교 농업생명환경대학 축산학과) ;
  • 김관석 (충북대학교 농업생명환경대학 축산학과) ;
  • 김종주 (영남대학교 생명공학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 윤두학 (농촌진흥청 국립축산과학원) ;
  • 강성호 (전북대학교 화학과 이화학연구소) ;
  • 정효일 (연세대학교 기계공학과) ;
  • 정일정 (한경대학교)
  • Received : 2009.07.01
  • Accepted : 2009.08.15
  • Published : 2009.08.01

Abstract

DNA marker information is used to identify or distinguish cattle breeds or individual animal. The purpose of this study was to apply Bovine Genotypes Kit Version 1.1/2.1 to bovine DNA samples (National Institute of Animal Science) taken from Australian / American beef (n=148), Holstein beef (n=170) and Hanwoo cattle (n=177) bred in Jeongeub, Jeonbuk, Korea, so that it could distinguish Hanwoo breed. The Bovine Genotype Kits consist of 16 ISAG MS markers, which were used to build a database of genotypes in each group. Genotyping results were analyzed using MS Tool kit and Phylip program to create phylogenetic tree. The GeneClass 2.0 was used to estimate breed identification. These analyses found that this kit had 100% capacity to distinguish Hanwoo beef, 95.3% capacity to differentiate Australian / American beef and 90% capacity to identify Korean Holstein steer beef. Hence, it is expected that 16 commercial microsatellite markers is useful to categorizegenetic characteristics of Hanwoo breed and also identify Hanwoo individuals and the origin of beef. In particular, it is expected that these markers will be advantageous in discriminating domestic Holstein beef from Australian / Americanbeef.

Keywords

Hanwoo;Microsatellite;Individual identification;Geneclass analysis;Phylogenetic analysis

Acknowledgement

Grant : Bovine Genotype Panel을 이용한 한우육 판별 및 판정 마커의 분석체계 수립, 한우 육량육질 조기선발용 DNA kit 산업화기술개발

Supported by : 농촌진흥청

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Cited by

  1. Comparison of the Microsatellite and Single Nucleotide Polymorphism Methods for Discriminating among Hanwoo (Korean Native Cattle), Imported, and Crossbred Beef in Korea vol.34, pp.6, 2014, https://doi.org/10.5851/kosfa.2014.34.6.763