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Studies on the Isolation and Identification of Xylanase and Mannanase Producing Aspergillus niger

Xylanase와 Mannanase를 생산하는 Aspergillus niger의 분리와 동정에 관한 연구

  • Received : 2009.08.04
  • Accepted : 2009.10.21
  • Published : 2009.10.01

Abstract

This study was undertaken to screen a high xylanase and mannanase producing microbes. In the first experiment, screening was undertaken against 50 samples of microorganisms having xylanase and mannanase activities from soil and fallen leaves. The screening process has focused on picking out fungi having high xylanase and mannanase activities under the solid-state fermentation. The xylanase and mannanase activities of 6 screened microbes were 0.9~1.6 unit/mL and 0.2~0.4 unit/mL, respectively, under the submerged fermentation condition. However, under the solid-state fermentation, xylanase and mannanase activities were 103.7~220.0 unit/g and 20.1~40.3 unit/g, respectively. Finally one microbe (E-3) was selected and its xylanase and mannanase activities were 197.3 unit/g and 39.9 unit/g, respectively. The morphological and molecular biological classification of E-3 showed 99% homology with the Aspergillus niger.

Keywords

Aspergillus niger;Xylanase;Mannanase

Acknowledgement

Supported by : 고품질친환경농축산물생산기술연구센터(GRRC)

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