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Screening and Isolation of Chitinase and Chitosanase Producing Microbes from the Feces of Korean Native Calves Medicated DFMs Including Chitin

키틴함유 DFMs 급여 한우송아지 분변내 키틴 및 키토산분해효소 생산 미생물 선발 및 동정

  • Kim, Tae-Il (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kwon, Eung-Gi (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyeong-Cheol (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Young-Moo (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Park, Byung-Ki (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Lee, Won-Kyu (Han Dong Co. Ltd) ;
  • Im, Seok-Ki (Hanwoo Experiment Station, National Institute of Animal Science, RDA)
  • 김태일 (농촌진흥청 국립축산과학원) ;
  • 권응기 (농촌진흥청 국립축산과학원) ;
  • 김형철 (농촌진흥청 국립축산과학원) ;
  • 조영무 (농촌진흥청 국립축산과학원) ;
  • 박병기 (농촌진흥청 국립축산과학원) ;
  • 이원규 ((주)한동) ;
  • 임석기 (농촌진흥청 국립축산과학원)
  • Received : 2009.09.04
  • Accepted : 2009.10.20
  • Published : 2009.10.01

Abstract

This study was carried out to screen and identify the chitinase and chitosanase producing microorganisms from the feces of calves medicated DFM sincluding chitin in order to do the immune fortification of Korean Native calves. Ten isolates were grown in the medium containing chitin and chitosan that had more than $10^5$ cfu/g in feces. Among these 10 strains, 2 strains (HANDI 110 and HANDI 309) had the chitinase activities and 2 strains (HANWOO and HANYOO) had the chitosanase activities in calves' feces. They showed no reaction in hemolysis tests by utilizing chitin and chitosan. The results from morphological, physicochemical and genetical identification indicated the HANDI 110 as a strain of Escherichia fergusonii, HANDI 309 was identified as a strain of Acinetobacter parvus, HANWOO was identified as a strain of Comamonas koreensis, and HANYOO as a strain of Chryseobacterium indologenes.

Keywords

Korean Native Calves;Chtinase;Chitosanase;Microbes;Feces;DFMs

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