Protein Fractionation of Whole Crop Silages, and Effect of Borate-phosphate Buffer Extraction on In vitro Fermentation Characteristics, Gas Production and Degradation

사료작물 사일리지의 단백질 분획 및 Borate-phosphate Buffer 추출이 In vitro 발효성상, Gas 발생 그리고 분해율에 미치는 효과

  • Shinekhuu, Judder (Department of Animal Science, Chungbuk National University) ;
  • Jin, Guang-Lin (Department of Animal Science, Chungbuk National University) ;
  • Ji, Byung-Ju (Department of Animal Science, Chungbuk National University) ;
  • Li, Xiangzi (Department of Animal Science, Yanbian University) ;
  • Oh, Young-Kyoon (National Institute of Animal Science, Rural Development Administration) ;
  • Hong, Seong-Ku (National Institute of Animal Science, Rural Development Administration) ;
  • Song, Man-Kang (Department of Animal Science, Chungbuk National University)
  • ;
  • 김광림 (충북대학교 농업생명환경대학 축산학과) ;
  • 지병주 (충북대학교 농업생명환경대학 축산학과) ;
  • ;
  • 오영균 (농촌진흥청 축산과학원) ;
  • 홍성구 (농촌진흥청 축산과학원) ;
  • 송만강 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2009.06.15
  • Accepted : 2009.10.08
  • Published : 2009.10.01


Protein fractionation was evaluated from whole crop silages of rye (RS), wheat (WS), triticale (TS), oat (OS), barley (BS), and rice straw silage (RSS), and in vitro trial was carried out to examine the effect of silage and extraction of soluble protein on fermentation characteristics, total gas production and degradation. Soluble protein of silages was extracted with borate-phosphate buffer, and fermentation characteristics, gas production and degradation of silages were estimated by incubating anaerobically the mixed solution of strained rumen fluid and artificial saliva (1:1, v/v) containing dried and ground silages placed in nylon bag at $39^{\circ}C$ up to 48h. Soluble protein (SP) content was lowest for RSS as 2.11% in total CP compared to those for other silages. Highest A fraction (NPN) was observed from RS (74.33% of total CP) while those from TS and RSS were relatively low (48%). B2 fraction was relatively higher for RS, RSS and WS than for TS and BS. $B_3$ fraction was lowest in WS among silages. C fraction (27.07) in RSS was higher than in other silages (1.40~9.93%). pH in incubation solution was increased (P<0.01~P<0.001) for extracted silages up to 12h but decreased (P<0.01) at 48h for non-extracted ones. Contents of ammonia-N (P<0.001) and total VFA (P<0.01~P<0.001) were higher for non-extracted silages than for extracted ones. Acetate proportion was increased (P<0.001) in buffer extracted silages while those of propionate and butyrate were decreased (P<0.001) up to 24h incubation. Increased (P<0.001) total gas production was obtained from non-extracted silages up to 12h while gas production was increased (P<0.01) in extracted ones thereafter. In vitro degradation of dry matter and CP was increased (P<0.001) in non-extracted silages but that of neutral detergent fiber was increased (P<0.001) in extracted ones without difference among silages. Difference in mean values of degradability for each silage prior to- and post extraction with borate buffer, however, was not found among silages. It may be concluded that high NPN content of silages may reduce the protein availability in silages and borate buffer soluble components in silages can stimulate the early stage of fermentation.


Whole crop silages;Protein fractionation;Fermentation;Gas production;In vitro degradation


Supported by : 농촌진흥청


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