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Karyotype of Jeju Horse; G-, C- and NOR-banding

제주재래마의 핵형분석; G-, C- 및 NOR-banding

  • Park, Jin-Sik (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Cho, Byung-Wook (Department of Animal Science, Pusan National University) ;
  • Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Jinju National University)
  • 박진식 (진주산업대학교 동물생명과학과) ;
  • 조병욱 (부산대학교 동물생명자원학과) ;
  • 손시환 (진주산업대학교 동물생명과학과)
  • Received : 2009.09.22
  • Accepted : 2009.10.21
  • Published : 2009.10.01

Abstract

This study was carried out to establish the standard karyotype of Jeju horse by G-, C- and AgNOR-banding patterns. Blood samples were collected from 37 Jeju horses and 24 Thoroughbred that had been raised at the National Institute of Subtropical Agriculture in Jeju. The lymphocytes were cultured in vitro and then chromosomes prepared. The diploid chromosome number of Jeju horse is 64, which consists of 31 pairs of autosomes and X, Y sex chromosomes. The Jeju horse has 13 pairs of metacentric/submetacentric and 18 pairs of acrocentric autosomes. The X chromosome is the fifth largest submetacentric, while the Y chromosome is one of the smallest acrocentric chromosomes. The G-banding pattern of Jeju horse chromosomes showed a light band at centromeres in all autosomes, and also exhibited a typical and identical banding pattern in each homologous chromosome. Overall chromosomal morphology and positions of typical landmarks of the Jeju horse were virtually identical to those of International Committee for the Standardization of the Domestic Horse Karyotype. C-bands of Jeju horse chromosomes appeared on centromeres of almost all autosomes, but chromosome 8 showed a heterochromatin heteromorphism. The NORs in Jeju horse chromosomes showed polymorphic patterns within breed, individuals and cells. By the AgNOR staining, the NORs were located at the terminal of p-arm on chromosome 1 and near centromeres on the chromosome 26 and 31. The mean number of NORs per metaphase was 4.68 in Jeju horse.

Keywords

Jeju horse;G-banding;C-banding;AgNORs;Karyotype

Acknowledgement

Supported by : 진주산업대학교

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