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A Comparison of Discriminating Powers Between 14 Microsatellite markers and 60 SNP Markers Applicable to the Cattle Identification Test

소 동일성 검사에 적용 가능한 14 Microsatellite marker와 60 Single Nucleotide Polymorphism marker 간의 판별 효율성 비교

  • Lim, Hyun-Tae (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University) ;
  • Seo, Bo-Yeong (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University) ;
  • Jung, Eun-Ji (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University) ;
  • Yoo, Chae-Kyoung (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University) ;
  • Yoon, Du-Hak (National Institute of Animal Science, R.D.A.) ;
  • Jeon, Jin-Tae (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University)
  • 임현태 (경상대학교 응용생명과학부(BK21)) ;
  • 서보영 (경상대학교 응용생명과학부(BK21)) ;
  • 정은지 (경상대학교 응용생명과학부(BK21)) ;
  • 유채경 (경상대학교 응용생명과학부(BK21)) ;
  • 윤두학 (농촌진흥청 국립축산과학원) ;
  • 전진태 (경상대학교 응용생명과학부(BK21))
  • Received : 2009.08.04
  • Accepted : 2009.10.15
  • Published : 2009.10.01

Abstract

When 14 microsatellite (MS) markers were applied in the identifying test for 480 Hanwoo, the discriminating power was estimated as $3.43{\times}10^{-27}$ based on the assumption of a random mating group (PI). This rate is 1,000 times higher than that of 60 single nucleotide polymorphism (SNP) markers. On the other hand, the power of the 60 SNP markers was estimated as $4.69{\times}10^{-20}$ and $8.02{\times}10^{-12}$ on the assumption of a half-sib mating group ($PI_{half-sibs}$) and a full-sib mating group ($PI_{sibs}$), respectively. These powers were 10 times and 10,000 times higher than those of the 14 MS markers. The results indicated that the total number of alleles (MS vs SNP = 146 vs 120) acted as a key factor for the discriminating power in a random mating population, and the total number of markers (MS vs SNP = 14 vs 60) was a dominant influence on the power in half-sib and full-sib populations. In the Hanwoo population, in which it was assumed that the entire population is the enormous half-sib group formed by the absolute genetic contribution of a few nuclear bulls, there will be only a 10 times difference in the discriminating power between the 14 MS markers and the 60 SNP makers. However, the probability of not excluding a candidate parent pair from the parentage of an arbitrary offspring, given that only the genotype of the offspring ($PNE_{pp}$) was 1,000 times higher as shown by the 14 MS markers than that by the 60 SNP markers. The strong points of SNP makers are the stability of the variation (low mutation rate) and automation of high-throughput genotyping. In order to apply these merits for the practical and constant Hanwoo identity test, research and development are required to set a cost-effective platform and produce a homemade apparatus for SNP genotyping.

Keywords

Microsatellite (MS);Single Nucleotide Polymorphism (SNP);Traceability;Discriminating power

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