Effect of Amino Acids and Organic Nitrogen Sources on Cyclosporin A Fermentation by Tolypocladium inflatum

Tolypocladium inflatum을 이용한 Cyclosporin A 발효에서 아미노산과 유기질소원의 영향

  • Kim, Jeong-Keun (Department of Chemical Engineering and Biotechnology, Korea Polytechnic University) ;
  • Lee, Byung-Kyu (Yuhan Research Institute) ;
  • Chang, Seog-Won (Bio RIC and Department of Medico-Life Science, Youngdong University) ;
  • Park, Yong-Deok (Bio RIC and Department of Medico-Life Science, Youngdong University) ;
  • Rho, Yong-Taek (Bio RIC and Department of Medico-Life Science, Youngdong University)
  • 김정근 (한국산업기술대학교 생명화학공학과) ;
  • 이병규 (유한양행 중앙연구소) ;
  • 장석원 (영동대학교 Bio RIC 및 의생명과학과) ;
  • 박용덕 (영동대학교 Bio RIC 및 의생명과학과) ;
  • 노용택 (영동대학교 Bio RIC 및 의생명과학과)
  • Received : 2008.07.30
  • Accepted : 2009.05.17
  • Published : 2009.06.28

Abstract

Cyclosporin, an immunosuppressant, is a representative group of biologically active secondary metabolites produced by the fungus Tolypocladium inflatum. The amount and ratio of cyclosporin derivatives in the culture broth are an important factors for the production of cyclosporin A and the purification in the industrial process. Therefore, we studied the effect of amino acids and complex organic nitrogen sources using Tolypocladium inflatum mutants on the productivity of cyclosporin A and the ratio of cyclosporin derivatives. Overproducing mutant YHC-004 having seven times higher productivity than mother strain's could be obtained through the artificial mutation by UV irradiation. The concentration and kind of organic nitrogens and amino acids shows the profound effect on the productivity of cyclosporin A and ratio of cyclosporin derivatives. As a result, it was possible to raise the productivity and the ratio of cyclosporin A up to 3,430 mg/L and 93% respectively, but on the other hand the other cyclosporin derivatives decreased less than 2% in the culture broth.

Keywords

Cyclosporin A;immunosuppressant;Tolypocladium inflatum;nitrogen source

Acknowledgement

Supported by : 영동대학교

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