Evaluation of Antimicrobial, Antithrombin, and Antioxidant Activity of Dioscorea batatas Decne

마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가

  • Kim, Jee-In (Dept. of Biological Science, Andong National University) ;
  • Jang, Han-Su (Gyeongbuk Institute for Bioindustry) ;
  • Kim, Jong-Sik (Dept. of Biological Science, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 김지인 (안동대학교 생명과학과) ;
  • 장한수 (경북바이오산업연구원) ;
  • 김종식 (안동대학교 생명과학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2009.04.14
  • Accepted : 2009.06.05
  • Published : 2009.06.28

Abstract

Yam (Dioscorea batatas Decne) has been recognized as healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, anti-mutagenic activity, and decrease of blood glucose and cholesterol level. In this study, the methanol extract and its organic solvent fractions were prepared from dried Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The 66.7% of methanol extract was fractioned into water residue, and most of total-polyphenol/total-flavonoids were found in ethylacetate fraction. This ethylacetate fraction at $500\;{\mu}g/disc$ concentration showed strong antibacterial activity against gram-negative and gram-positive bacteria, except Staphylococcus aureus. Antifungal activity was not observed in methanol extract and its fractions. But, strong antithrombin activity was found in methanol extract. At 4.8 mg/mL concentration, thrombin time was 99.5 sec, which is coincides with 30% activity of aspirin, a commercial available antithrombosis agent. The ethylacetate fraction showed strong DPPH scavenging activity ($IC_{50}$ of $80.5\;{\mu}g/mL$), compared to vitamin C ($IC_{50}$ of $15.2\;{\mu}g/mL$). Also, the ethylacetate fraction showed strong SOD-like activity and reducing power, which are coincide with 43% of vitamin C and 82.7% of butylated hydroxytoluene activity, respectively.

Keywords

Antimicrobial;antioxidant;antithrombosis;Dioscorea batatas Decne;Yam

Acknowledgement

Supported by : 농림부

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