Evaluation of Antimicrobial, Antithrombin, and Antioxidant Activity of Dioscorea batatas Decne

마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가

  • Kim, Jee-In (Dept. of Biological Science, Andong National University) ;
  • Jang, Han-Su (Gyeongbuk Institute for Bioindustry) ;
  • Kim, Jong-Sik (Dept. of Biological Science, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 김지인 (안동대학교 생명과학과) ;
  • 장한수 (경북바이오산업연구원) ;
  • 김종식 (안동대학교 생명과학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2009.04.14
  • Accepted : 2009.06.05
  • Published : 2009.06.28


Yam (Dioscorea batatas Decne) has been recognized as healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, anti-mutagenic activity, and decrease of blood glucose and cholesterol level. In this study, the methanol extract and its organic solvent fractions were prepared from dried Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The 66.7% of methanol extract was fractioned into water residue, and most of total-polyphenol/total-flavonoids were found in ethylacetate fraction. This ethylacetate fraction at $500\;{\mu}g/disc$ concentration showed strong antibacterial activity against gram-negative and gram-positive bacteria, except Staphylococcus aureus. Antifungal activity was not observed in methanol extract and its fractions. But, strong antithrombin activity was found in methanol extract. At 4.8 mg/mL concentration, thrombin time was 99.5 sec, which is coincides with 30% activity of aspirin, a commercial available antithrombosis agent. The ethylacetate fraction showed strong DPPH scavenging activity ($IC_{50}$ of $80.5\;{\mu}g/mL$), compared to vitamin C ($IC_{50}$ of $15.2\;{\mu}g/mL$). Also, the ethylacetate fraction showed strong SOD-like activity and reducing power, which are coincide with 43% of vitamin C and 82.7% of butylated hydroxytoluene activity, respectively.


Antimicrobial;antioxidant;antithrombosis;Dioscorea batatas Decne;Yam


Supported by : 농림부


  1. Kang, T. H., S. Choi, T. Lee, M. Son, J. Park, and S. Y. Kim. 2008, Characteristics of antidiabetic effect of Dioscorea rhizoma (2) - prevention of diabetic neuropathy by NGF induction. Kor. J. Food Nutr. 21: 430-435
  2. Kim, J. O., M. J. Jung, H. J. Choi, J. T. Lee, A. K., Lim, J. H. Hong, and D. I. Kim. 2008. Antioxidant and biological activity of hot water and ethanol extracts from Phellinus linteus. J. Kor. Soc. Food Sci Nutr. 37: 684-690 https://doi.org/10.3746/jkfn.2008.37.6.684
  3. Park, C. S., K. M. Yang, and M. L. Kim. 2006. Functional properties of medicinal plant extracts. Kor. J. Food. Cookery Sci. 23: 720-727
  4. Ryu, H. Y., E. J. Kum, K. H. Bae, Y. K. Kim, I. S. Kwon, and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and antithrombosis activity of Korean traditional liquors. Kor. J. Microbiol. Biotechnol. 35: 310-315
  5. Ryu, H. Y., Y. S. Kim, S. J. Park, B. H. Lee, S. T. Kwon and H. Y. Sohn. 2006. Isolation and characterization of yamputrefactive psychrotrophic bacteria from rotted yam. Kor. J. Microbiol. Biotechnol. 34: 109-114
  6. Shin, S. R. 2004. Changes on the components of yam snack by processing methods. Kor. J. Food Preservation 11, 516-521
  7. Sohn, H. Y. H. Y. Ryu, Y. Jang, H. S. Jang, Y. M. Park, and S. Y. Kim. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor. J. Microbiol. Biotechnol. 36: 195-200
  8. Ahn J. H., K. H. Son, H. Y. Sohn, and S. T. Kwon. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Kor. J. Plant Biotechnol. 32: 317-223
  9. Han, Y. N., S. H. Hahn, and I. R. Lee. 1990. Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis. Kor. J. Pharmacogn. 21: 274-283
  10. Kwon, C. S., I. S. Son, H. Y. Shim, I. S. Kwun, and K. M. Chung. 1999. Effects of yam on lowering cholesterol level and its mechanism. Kor. J. Food Nutr. 32: 637-643
  11. Sohn, H. Y., K. H. Son, C. S. Kwon, G. S. Kwon, and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11: 666-672 https://doi.org/10.1016/j.phymed.2003.09.005
  12. Lee, B. Y., and H. K. Kim. 1998. Quality properties of korean yam by various drying methods. Kor. J. Food Sci. Technol. 30: 877-882
  13. Kwon, C. S., H. Y. Sohn, S. H. Kim, J. H. Kim, K. H. Son, J. S. Lee, J. K. Lim, and J. S. Kim. 2003. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67, 1451-1456 https://doi.org/10.1271/bbb.67.1451
  14. Sohn, H. Y., C. S. Kwon, K. H. Son, G. S. Kwon, H. Y. Ryu, and E. J. Kum. 2006. Antithrombin and thrombosis prevention activity of buckwheat seed, Fagopyrum esculentum Moench. J. Kor. Soc. Food Sci. Nutr. 35: 132-138 https://doi.org/10.3746/jkfn.2006.35.2.132
  15. Hironaka, K., K. Takada, and K. Ishilbashi. 1990. Chemical composition of mucilage of chinese yam. Nippon Shokuhin Kogyo Gakkaishi 37: 48-51 https://doi.org/10.3136/nskkk1962.37.48
  16. Na, M., B. S. Min, R. B. An, W. Jin, Y. H. Kim, K. S. Song, Y. H. Seong and K. Bae. 2004. Effect of the rhizomes of Astilbe chinensis on UVB-induced inflammatory response. Phytother. Res. 18: 1000-1004 https://doi.org/10.1002/ptr.1599
  17. Kim M. W. 2001. Effects of H2O-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin-induced diabetic rats. Kor. J. Soc. Food Cookery Sci. 17, 344-352
  18. Park, C. S. 2005. Antioxidant and nitrite scavenging ability of medicinal plant extracts. Kor. J. Food Preserv. 12: 631-636
  19. Ryu, H. Y., S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Control of yam putrefactive psychrotrophic bacterium using clove oil and preparation of functional Fresh-cut. Kor. J. Microbiol. Biotechnol. 35: 66-72
  20. Kim, E. Y, I. H. Baik, J. H. Kim, S. R. .Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36: 333-338
  21. Kum, E. J., S. J. Park, B. H. Lee, J. S. Kim, K. H. Son, and H. Y. Sohn. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscroea batatas decne. J. Life Sci. 16, 647-652 https://doi.org/10.5352/JLS.2006.16.4.647
  22. Kwon, E. G., E. M. Choe, and S. J. Gu. 2001. Effects of mucilage from yam (Dioscorea batatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Kor. J. Food Sci. Technol. 33, 795-801
  23. Marklund, S., and G. Marklund. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  24. Kang, T. H., S. Choi, T. Lee, M. Son, and S. Y. Kim. 2008, Characteristics of antidiabetic effect of Dioscorea rhizoma (1) - Hypoglycemic effect. Kor. J. Food Nutr. 21: 425-429
  25. Lee I.-S., S.-Y. Chung, C.-S. Shim, and S. J. Koo. 1995. Inhibitory effects of yam (Dioscorea batatas DECNE) extracts on the mutagenicity. Kor. J. Soc. Food Sci.. 11: 351-355
  26. Shin, K. O., J. R. Jeon, J. S. Lee, J. Y. Kim, C. H. Lee, S. D. Kim, Y. S. Yu, and D. H. Nam. 2006. Lactic acid fermentation of chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model. Biotechnol. Bioprocess Eng. 11: 240-244 https://doi.org/10.1007/BF02932037