Characterization of Antimicrobial Substance Produced by Lactobacillus sp. HN 235 Isolated from Pig Intestine

돼지 장관으로부터 분리한 Lactobacillus sp. HN 235 균주가 생산하는 항균물질의 특성

  • Shin, Myeong-Su (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.) ;
  • Han, Sun-Kyung (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Ji-Hyun (College of Veterinary Medicine, Chungbuk National University) ;
  • Ji, Ae-Ran (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.) ;
  • Kim, Kyeong-Su (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.) ;
  • Lee, Wan-Kyu (College of Veterinary Medicine, Chungbuk National University)
  • 신명수 ((주)오비티 한국생명과학연구소) ;
  • 한선경 (충북대학교 수의과대학) ;
  • 최지현 (충북대학교 수의과대학) ;
  • 지애란 ((주)오비티 한국생명과학연구소) ;
  • 김경수 ((주)오비티 한국생명과학연구소) ;
  • 이완규 (충북대학교 수의과대학)
  • Received : 2009.04.14
  • Accepted : 2009.05.18
  • Published : 2009.06.28


In order to develop probiotics which may be a viable alternative of antibiotic use in pig industry, five bacterial strains (Lactobacillus sp. HN 52, 92, 98, 235 and AP 116) possessing antimicrobial properties were selected from 500 strains isolates of pig intestines. The bacteriocin produced by Lactobacillus sp. HN 235 displayed a relative broad spectrum of inhibitory activity against all Enterococcus strains, Pseudomonas aeruginosa, Listeria monocytogenes and Clostridium perfringens using the spot-on-lawn method. The production of antimicrobial substance started in the middle of the exponential growth phase, reached maximum levels (6,400 AU/mL) in the stationary phase, and then declined. Bacteriocin activity remained unchanged after 30 min of heat treatment at $95^{\circ}C$ and stable from pH 2.0 to 10 for 1 h, or exposure to organic solvents; however, it diminished after treatment with proteolytic enzymes. The molecular weight of the bacteriocin was about 5 kDa according to a tricine SDS-PAGE analysis.


Antimicrobial substance;bacteriocin;lactic acid bacteria;probiotics;pig


Supported by : 충북대학교


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