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Detection of a Large White-Specific Duplication in D-loop Region of the Porcine MtDNA

돼지 mtDNA D-loop 지역의 Large White 특이 중복현상 탐지

  • Kim, Jae-Hwan (National Institute of Animal Science, R. D. A.) ;
  • Han, Sang-Hyun (National Institute of Animal Science, R. D. A.) ;
  • Lee, Sung-Soo (National Institute of Animal Science, R. D. A.) ;
  • Ko, Moon-Suk (National Institute of Animal Science, R. D. A.) ;
  • Lee, Jung-Gyu (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, In-Cheol (National Institute of Animal Science, R. D. A.)
  • 김재환 (농촌진흥청 국립축산과학원) ;
  • 한상현 (농촌진흥청 국립축산과학원) ;
  • 이성수 (농촌진흥청 국립축산과학원) ;
  • 고문석 (농촌진흥청 국립축산과학원) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 조인철 (농촌진흥청 국립축산과학원)
  • Published : 2009.04.30

Abstract

The entire D-loop region of the porcine mitochondrial DNA (mtDNA) was amplified from six pig breeds (Landrace, Duroc, Large White, Korean native pig, Berkshire, and Hampshire) using a primer set designed on the basis of reported porcine mtDNA sequences. From analyses through cloning, DNA sequencing and multiple sequence alignment, an 11-bp (TAAAACACTTA) duplication was observed after known tandem repetition in the D-loop region, which promoted hetroplasmy in mtDNA. Although the existence of the 11-bp duplication has been previously reported in Duroc and Japanese native pigs, there have not been any attempts to know the characteristics of this duplication in other breeds so far. A 150 bp fragment containing the 11-duplication was amplified and typed by polyacrylamide gel electrophoresis (PAGE). All Large Whites had two duplication units and Duroc showed heteromorphic patterns, 11.2% (9/80) of the animals had the 11-bp duplication in total. On the other hand, Landrace, Berkshire, Hampshire and Korean native pigs were non-duplicated. This result showed that the 11-bp duplication could be used as a breed-specific DNA marker for distinguishing pure Landrace and Large White breeds.

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