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Media Characteristics of PVA-derivative Hydrogels Using a CGA Technique

CGA 제조기법을 응용한 PVA 하이드로젤의 담체 특성

  • Yoon, Mi-Hae (Department of Energy & Environmental Engineering Soonchunhyang University) ;
  • Kwon, Sung-Hyun (Department of Marine Environmental Engineering/Institute of Marine Industry Gyeongsang National University) ;
  • Cho, Dae-Chul (Department of Energy & Environmental Engineering Soonchunhyang University)
  • 윤미해 (순천향대학교 에너지환경공학과) ;
  • 권성현 (경상대학교 해양환경공학과 (해양산업연구소)) ;
  • 조대철 (순천향대학교 에너지환경공학과)
  • Published : 2009.03.31

Abstract

We manufactured PVA-derived hydrogels using a foam generation technique that has been widely used to prepare colloidal gas aphrons(CGA). These gels were differentiated to the conventional gels such as for medical or pharmaceutical applications, which have tiny pores and some crystalline structure. Rather these should be used in de-pollution devices or adhesion of cells or biomolecules. The crosslinkers used in this work were amino acid, organic acid, sugars and lipids(vitamins). The structures of the gels were observed in a scanned electron microscope. Amino acids gels showed remarkably higher swelling ratios probably because their typical functional groups help constructing a highly crosslinked network along with hydrogen bonds. Boric acid and starch would catalyze dehydration while structuring to result in much lower water content and accordingly high gel content, leading to less elastic, hard gels. Bulky materials such as ascorbic acid or starch produced, in general, large pores in the matrices and also nicotinamide, having large hydrophobic patches was likely to enlarge pore size of its gels as well since the hydrophobicity would expel water molecules, thus leading to reduced swelling. Hydrophilicity(or hydrophobicity), functional groups which are involved in the reaction or physical linkage, and bulkiness of crosslinkers were found to be more critical to gel's cross linking structure and its density than molecular weights that seemed to be closely related to pore sizes. Microscopic observation revealed that pores were more or less homogeneous and their average sizes were $20{\mu}m$ for methionine, $10-15{\mu}m$ for citric acid, $50-70{\mu}m$ for L-ascorbic acid, $30-40{\mu}m$ for nicotinamide, and $70-80{\mu}m$ for starch. Also a sensory test showed that amino acid and glucose gels were more elastic meanwhile acid and nicotinamide gels turned out to be brittle or non-elastic at their high concentrations. The elasticity of a gel was reasonably correlated with its water content or swelling ratio. In addition, the PVA gel including 20% ascorbic acid showed fair ability of cell adherence as 0.257mg/g-hydrogel and completely degraded phenanthrene(10 mM) in 240 h.

Keywords

PVA;Hydrogel;CGA technique;Cell adherence;Cross-linker;Amino acid;Organic acid;Lipid;Saccharide

References

  1. Kim C. H., Lee J. B., Kim D. H., Hwang J. M., Cho C. S., Choi Y. H., Chung D. W., 1999, Synthesis and characteristics of photo-crosslinkable hydrogel for microbial immobilization, J. Ind. Eng. Chem, 10(6), 852-856
  2. Kim G. W., 2005, Hyaluronic Acid-Based Hydrogel Synthesis and Its Applications in Bone and Vascular Tissue Regenerations, Dept. of Chemical Engineering, Seoul National University, Seoul
  3. Enscore D. J., Hopfenberg H. B., Stannett V. T., 1977, Effect of particle size on the mechanism controlling n-hexane sorption in glassy polystyrene microspheres, J. Polym. Sci., 18(8), 793-800 https://doi.org/10.1016/0032-3861(77)90183-5
  4. Hariharan D., Peppas N. A., 1994, Modelling of water transport in ionic hydrophilic polymers, J. Polym. Sci, Polym, Phys., 32, 1093-1103 https://doi.org/10.1002/polb.1994.090320614
  5. Kim M. R., 2002, Temperature-responsive and degradable hyaluronic acid/pluronic composite hydrogels for controlled release of human growth hormone, M. S. Dissertation, Dept. Biological Science. KAIST, Daejeon
  6. Park C. H., Chung I. S., 1999, Concentration of virus for vaccine development using pH/temperaturesensitive hydrogel, M. S. Dissertation, Dept. Chem Eng., Kyung Hee University, Seoul
  7. Yoo M. K., Sung Y. K., 1998, Formation of complex between polyelectrolytes and pH/temperature sensitive copolymers, J. Chem. Sci., 42(1), 84-91
  8. Feil H., Bae Y. H., Feijen J., Kim S. W., 1992, Mutual influence of pH and temperature on the swelling of ionizable and thermosensitive hydrogels, Macromolecules, 25, 5528-5530 https://doi.org/10.1021/ma00046a063
  9. Baker J. P., Siegel R. A., 1996, Hysteresis in the glucose permeability versus pH characteristic for a responsive hydrogel membrane, Macromol. Rapid Comm., 17, 409-415 https://doi.org/10.1002/marc.1996.030170607
  10. Wu J. Z., Sassi A. P., Blanch H. W., Prausniz J. M., 1996, Partitioning of proteins between an aqueous solution and a weakly-ionizable ployelectrolyte hydrogel, Polymer, 37, 4803-4808 https://doi.org/10.1016/S0032-3861(96)00326-6
  11. Park T. G., Hoffaman A. S., 1992, Synthesis and characterization of pH-and/or temperature-sensitive hydrogels, J. Appl. Pol. Sci., 46, 659-671 https://doi.org/10.1002/app.1992.070460413
  12. Jung S. Y., 2004, Studies on the preparation and characterization of Poly(vinyla1cohol)-Poly(acrylicacid) hydrogel, M. S. Dissertation, Dept. of Chemical Engineering, Hannam University, Daejeon
  13. Donaldson E., Cuy J., Nair P., Ratner B., 2005, Poly(vinyl alcohol)-Amino acid hydrogels fabricated into tissue engineering scaffolds by colloidal gas aphron technology, J. Macromol. Symp. 227, 115-122 https://doi.org/10.1002/masy.200550911
  14. Sebba F., 1987, Foams and Biliquid Foams: Aphrons, John Wiley & Sons, Hoboken, New Jersey
  15. Yoon M. H., Cho D., 2007, A study on preparation of colloidal gas aphrons and stability, J. Kor. Soc. Environ. Eng., 29(6), 670-677