RAG-1 유전자의 염기서열 분석에 의한 각시붕어 Rhodeus uyekii와 떡납줄갱이 R. notatus 잡종의 동정

Genetic Identification of Hybrids between Rhodeus uyekii and R. notatus by Sequence Analysis of RAG-1 Gene

  • 윤영은 (순천향대학교 해양생명공학과) ;
  • 이일로 (순천향대학교 해양생명공학과) ;
  • 박상용 (순천향대학교 해양생명공학과) ;
  • 강언종 (국립수산과학원 남부내수면연구소) ;
  • 김응오 (국립수산과학원 남부내수면연구소) ;
  • 양상근 (국립수산과학원 남부내수면연구소) ;
  • 남윤권 (부경대학교 양식학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Yun, Young-Eun (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kang, Eon-Jong (Southern Regional Inland Research Institute, NFRDI) ;
  • Kim, Eung-O (Southern Regional Inland Research Institute, NFRDI) ;
  • Yang, Sang-Keun (Southern Regional Inland Research Institute, NFRDI) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 발행 : 2009.02.25

초록

한국 고유종인 각시붕어 R. uyekii와 떡납줄갱이 R. notatus로부터 유도된 정교배 및 상반교배 잡종어류의 분자생물학적 동정을 위하여 핵에서 encoding되는 RAG-1 유전자의 염기서열 분석을 실시하였다. 분석된 863 bp의 염기서열 중 각시붕어와 떡납줄갱이 사이에는 총 13개의 위치에서 염기서열 변이가 탐색되었다. 잡종어류의 RAG-1 유전자 염기서열을 분석한 결과 모계와 부계의 염기서열 차이를 보인 13개의 변이 부분에서 부모의 염기서열을 다같이 반영하는 double peaks 패턴을 보였으나 정교매체(UN 유전형)와 상반교배체(NU 유전형) 간의 염기서열 차이는 관찰되지 않았다.

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