Korean Mistletoe Viscum album coloratum Induces Specific and Non-Specific Immune Responses in Japanese Eel Anguilla japonica

  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Rhow, Jin-Goo (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Min-Soon (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Yoon, Jong-Man (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Gyung-Il (Department of Aquatic Life Medicine, Kunsan National University)
  • Published : 2009.02.25


Effects of Korean mistletoe extracts (KM-110), Viscum album coloratum on the specific and non-specific immune responses of Japanese eel Anguilla japonica were examined. The optimal concentration not showing toxicity of KM-110 was determined to $30-40{\mu}g/ml$ in vitro and $100{\mu}g$/100 g of fish in vivo. Even $1000{\mu}g$ of KM-110/100 g of fish did not show any clinical problem in fish though the levels of toxic parameters were slightly increased. In terms of antibody production, KM-110 significantly elicited more antibody production than FCA or $\beta$-glucan. $\beta$-glucan plus KM-110 group synergistically enhanced antibody production. There was no significant difference between KM-110 and KM-110 plus $\beta$-glucan group. The ROI production by head kidney (HK) leucocytes of eel injected with 500 or $1000{\mu}g$ KM-110 was significantly (P<0.05) enhanced than the control and FCA-treated group. Maximum increase in the NBT reduction value was observed in $1000{\mu}g$ KM-110 group but no significant difference was found between 500 and $1000{\mu}g$ KM group. The level of serum lysozyme activity was significantly (P<0.05) higher in the 500 and $1000{\mu}g$ KM-110- or FCA-treated group than in the control and $200{\mu}g$ KM-110 group. The phagocytic activities of HK leucocytes isolated from eel injected with 500 and $1000{\mu}g$ KM-110 were significantly (P<0.05) higher than $200{\mu}g$ KM-110 and PBS-injected control group. Korean mistletoe appeared to be a good activator of the specific and non-specific immune responses of Japanese eel.


  1. Bliznakov, E. G., A. D. Adler, 1972. Nonlinear response of the reticuloendothelial system upon stimulation. Pathol. Microbiol., 38, 393-410
  2. Cameron, J. S., K. E. Hoffmann, C. Zia, H. M. Hemmett,A. Kronsteiner, C. M. Lee, 2003. A role for nitric oxide in hypoxiainduced activation ofcardiac KATP channels in goldfish (Carassius αμratus). J. Exp. Biol., 206, 4057-4065 https://doi.org/10.1242/jeb.00655
  3. Chen, D and A. J. Ainsworth, 1992. Glucan administration potentiates immune defense mechanisms of channel catfish, Ictalurns punctauts Rafinesque. J. Fish Dis., 15, 295-304 https://doi.org/10.1111/j.1365-2761.1992.tb00667.x
  4. Engstad, R., B. Robertsen, E. Frivold, 1992. Yeast glucan induces increase in lysoηme and complement-mediated haemolytic activity in Atlantic salmon blood. Fish Shellfish immunol. 2, 287-297 https://doi.org/10.1016/S1050-4648(06)80033-1
  5. Hajto, T., 1986. Immunomodulatory effect of Iscador: a Viscum album preparation. Oncology, 43, (suppl. 1), 51-61 https://doi.org/10.1159/000226420
  6. Lyu, S. Y., S. M. Park, B. Y. Choung, W. B. Park, 2000. Comparative study of Korean (Viscum album var. coloratum) and European mistletoe (Viscum album). Arch. Pharm. Res., 23, 592-598 https://doi.org/10.1007/BF02975247
  7. Mannel, D. N., H. Becker, A. Gundt, H. Franz, 1991. Induction of tumor necrosis factor expression by a lectin from Viscum album. Cancer lmmunol. Immunother., 33, 177-182 https://doi.org/10.1007/BF01756139
  8. Mulero, V., J. Meseguer, 1998. Functional characterisation of a macrophage-activating factor produced by leucocytes of gilthead seabream (Sparns aurata L.). Fish & Shellfish Immunol., 8, 143-156 https://doi.org/10.1006/fsim.1997.0127
  9. Secombes, C. J., S. Chung, A. H. Jeffries, 1988. Superoxide anion production by rainbow trout macrophages detected by the reduction of ferricytochrome C. Dev. Comp. Immunol., 12, 201-206 https://doi.org/10.1016/0145-305X(88)90038-9
  10. Weis, W. I., M. E. Taylor, K. Drichamer, 1998. The C-type lectin superfamily in the immune system. Immunol. Rev., 163, 19-34 https://doi.org/10.1111/j.1600-065X.1998.tb01185.x
  11. Singru, P. S., A. J. Sakarharkar, N. Subhedar, 2003. Neuronal nitric oxide synthase in the olfactoη system of an adult teleost fish Oreochromis mossambicus. Brain Res., 11, 157-168
  12. Dianoux, A. C., P. Jolles, 1969. Differences in the behavior of chicken and goose egg white lysozymes against Micrococcus lysodeikticus. Bull. Soc. Chim. Biol., 51, 1559-1564
  13. Verlhac, V., J. Gabaudan, A. Obach, W. Schuep, R. Hole, 1996. Influence of dietary glucan and vitamin D on non-specifici responses of rainbow trout (Oncorhynchus mykiss). Aquaculture, 143, 123-133 https://doi.org/10.1016/0044-8486(95)01238-9
  14. Siwicki, A. K., M. Cossarini-Dunier, M. Studnicka, A. Demael, 1990. In vivo effect of the organophosphoms insecticide trichlorphon on immune response of carp (Cyprinus carpio). II. Effect of high doses of trichlorphon on nonspecific immune response. Ecotoxicol. Environ. Saf., 19, 99-105 https://doi.org/10.1016/0147-6513(90)90084-I
  15. Raa, J. 1996. The use of immunostimulatory substances in fish and shellfish farming. Rev. Fish Sci., 4, 229-288 https://doi.org/10.1080/10641269609388587
  16. Anderson, D. P., O, Jeney, 1992. Immunostimulants added to inected Aeromonas salmonicida bacterin enhance the defense mechanisms and protection on rainbow trout (Oncorhynchus mykiss). Vet. Immunol. Immunopathol., 34, 379-389 https://doi.org/10.1016/0165-2427(92)90177-R
  17. Bagni, M., N. Romano, M. G, Finoia, L. Abelli, G, Scapigliate, P. G Tiscar. 2005. Short- and long-term effects of a dietary yeast b-glucan (Macrogard) and alginic acid (Ergosan)preparation on immune response in sea bass (Dicentrarchus labrax). Fish & Shellfish Immunol., 18, 311-25 https://doi.org/10.1016/j.fsi.2004.08.003
  18. Midtlyng, P. J., L. J. Reitan, A. Lillehaug, A. Ramstad, 1996. Protection, immune responses and side effects in Atlantic salmon(Salmo salar L.) vaccinated against furunculosis by different procedures. Fish Shellfish Immunol. 6, 599-613 https://doi.org/10.1006/fsim.1996.0055
  19. Babior, B. M., 1984. Oxidants from phagocytes: agents of defcnse and destruction. Bolld, 64, 959-966
  20. Anderson, D. P., 1996. Environmental factors on fish health:immunological aspects. In: Iwama G, Nakanishi T, editors. The fish immune system: organism, pathogen, and environment. San Diego, CA: Academic Press, p. 289-310
  21. Vasta, G. R., H. Ahmed, N. E. Fink, M. T. Elola, A. G. A. M. Snowden, E. W. Odom, 1994. Animallectins as selflnon-self recongnition molecules. Biochemical and genetic approaches to understanding their biological roles and evolution. Ann. N.Y. Acad. Sci., 15, 55-73
  22. Kuttan, G. D., M. Vasudevan, R. Kuttan, 1992. Tumor reducing activity of an isolated active ingredient from mistletoe extract and its possible mechanism of aaion. J. Exp. Clin. Cancer Res., 11, 7-12
  23. Peddie, S., J. Zou, C. J. Secombes, 2002. Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan. Vet. Immunol. Immunopathol., 86, 101-113 https://doi.org/10.1016/S0165-2427(02)00019-3
  24. Sahoo, P. K., S. C. Mukherjee, 2001. Effect of dietary $\beta$-l,3 glulcan on immune responses and disease resistance of healthy and aflatoxin B l-induced immunocompromised rohu (Labeo rohita Hamilton). Fish & shellfish immunol., 11, 683-695 https://doi.org/10.1006/fsim.2001.0345
  25. Castro, R., N. Couso, A. Obach, J. Lamas, 1999. Effect of different beta-glucans on the respiratory burst of turbot (Psetta maxima) and gilthead rainbow trout (Sparns aurata) phagocytes. Fish & Shellfish Immunol., 9, 529-541 https://doi.org/10.1006/fsim.1999.0210
  26. Ellis, A. E., 1999. Immunity to bacteria in fish. Fish & Shellfish Immunol., 9, 291-308 https://doi.org/10.1006/fsim.1998.0192
  27. Gialdroni-Grassi, G., C. Grassi, 1985. Baαerial products as immunomodulating agents. Int. Arch .Allergy Appl. Immunol. 76, (Supp.l): 119-127 https://doi.org/10.1159/000233742
  28. Fulton, J. F., 1957. George Sarton and the history of medicine. Science, ;48, 311-314
  29. Holmskov, U., R. Malhotra, R. B. Sim, J. C. Jensenius, 1994. Collectins: collagenous C-type lectins of the innate immune defense system. Immunol. Today, 15, 67-74 https://doi.org/10.1016/0167-5699(94)90136-8
  30. Yoon, T. J., T. C. Yoo, T. B. Kang, E. Her, K. Kim, I. Azuma, J. B. Kim, 2001. Cellualar and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum). Int. Immunopharmacol., 1, 881-889 https://doi.org/10.1016/S1567-5769(01)00024-8
  31. Mertzer, G. H. H., A. Franz, B. Kindt, Fahlbusch, J. Sus, 1985. The in vitro activity of lectin 1 from mistletoe (ML 1) and its isolated A and B chains in functions of macrophages and polymorphonuclear cells. Immunobiol., 169, 461-471 https://doi.org/10.1016/S0171-2985(85)80002-4
  32. Sakai, M. 1999. Current research status offish immunostimulants. Aquaculture, 172, 63-92 https://doi.org/10.1016/S0044-8486(98)00436-0
  33. Kenyon, C. J., A. McKeever, J. A. Oliver, I. W. Henderson, 1985. Control of renal and adrenocortical function by the reninangiotensin system in two euryhaline teleost fishes. Gen. Comp. Endocrinol., 58, 93-100 https://doi.org/10.1016/0016-6480(85)90140-6
  34. Mueller, E. A., F. A. Anderer, 1990. Viscum album oligosac-chaaride activating human natural cytotoxicity is an interferon garnma inducer. Canaer Immunol. Immunother., 32, 221-227 https://doi.org/10.1007/BF01741704
  35. Daly, J. G., A. R. Moore, G. Olivier, 1995. A colorimetric assay for the quantivication of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis. Fish & Shellfish Immunol., 5, 265-273 https://doi.org/10.1006/fsim.1995.0026
  36. Lu, J. 1997. Collectins: collectors of microorganisms for the innate immune system. Bioassays, 19, 509--518 https://doi.org/10.1002/bies.950190610
  37. Santarem, M., B. Novoa, A. Figueras, 1997. Effect of beta-glucans on the non-specific immune responses of turbot (Scophthalmus L.) Fish & shellfish immunol., 7, 429-437 https://doi.org/10.1006/fsim.1997.0096
  38. Ni, Y., I. Tizard, 1993. Lectin-carbohydrate interaction in the immune system. Vet. Immunol. Immunopathol., 55, 205-223