Physiological Responses of Starry Flounder Platichthys stellatus during Freshwater Acclimation with Different Speeds in Salinity Change

염분변화 속도를 달리한 담수순화 과정에서 강도다리 Platichthys stellatus의 생리학적 반응

  • Kim, Young-Soo (Department of Aquaculture, Pukyong National University) ;
  • Do, Yong-Hyun (Department of Aquaculture, Pukyong National University) ;
  • Min, Byung-Hwa (Department of Aquaculture Management, National Fisheries Research and Development Institute) ;
  • Lim, Han-Kyu (Department of Aquaculture Management, National Fisheries Research and Development Institute) ;
  • Lee, Bok-Kyu (Department of Biology, Dongeui University) ;
  • Chang, Young-Jin (Department of Aquaculture, Pukyong National University)
  • Published : 2009.02.25

Abstract

Physiological responses (hematological factors, cortisol, glucose, osmolality, $Na^+$, $K^+$ and $Cl^-$) in starry flounder Platichthys stellatus were investigated during freshwater acclimation in the conditions of different speeds in salinity change with acute-decrease (AD) or stepwise-decrease (SD I and II). In AD of acute-decrease salinity, hematocrit (Ht), red blood cell (RBC) and hemoglobin (Hb) were rapidly increased more than SD I of stepwise-decrease salinity. But in case of SD II, Ht, RBC and Hb were no significant difference from beginning to end of this experiments. In AD, cortisol level significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.7{\pm}0.2{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\mu}g/mL$) at 10 days. In SD I, cortisol level was significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.6{\pm}0.6{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\pm}0.4{\mu}g/mL$) at 10 days. In SD II, cortisol level was a little increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $10.5{\pm}2.5$, $10.8{\pm}5.6{\mu}g/mL$ at 6, 12 hours and recovered to the basal level at 48 hours. Glucose level of AD, SD I, II were no significant difference from beginning to end of this experiments. Osmolality was $286.8{\pm}3.3\;mOsm/kg$ at the beginning. In SD II of stepwise-decrease, osmolality was no significant difference during rearing in freshwater (FW). But AD of stepwise-decrease and SD I of stepwise-decrease, osmolality was a little decreased end of this experiments. In AD of acute-decrease, only $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased. In case of SD I, $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased.

References

  1. Davis, K. B. and N. C. Parker, 1990. Physiological stress in striped bass: E:ffect of acclimation temperature. Aquaιulture,91, 349-358
  2. Eddy, F.B., 1981. Effects of stress on osmotic and ionic regulation in fish. (in) A.D. Pickering (ed)., Stress and Fish. Academic Press, London, p.77-102
  3. Kim, M. J., S. C. Chung and C. B. Song, 2004. Effect of salínity on growth and survival of olivε flounder, Paralichthys olivaceus. Korean J. Ichthyol., 16, 100-106. (in Korean)
  4. Mazeaud,M., F. Mazeaud and E. M, Donaldson. 1977. Primary and secondary effects of stress in fish: Somc new data with a general review. Trans. Ame. Fish. Soc., 106, 201-212 https://doi.org/10.1577/1548-8659(1977)106<201:PASEOS>2.0.CO;2
  5. Pickering, A. D. 1987. Stress responses and disease resistance in farmed fish. Fish diseases, a treat to the intemational fish farming industry. Conference 3, Aqua Nor, Trondheim, Norway
  6. Chang, Y. J. and J. W. Hur, 1999. Physiological responsεs of grey muIlet (Muhil cephalus) and Nile tilapia (Oreochromis niloticus) by rapid changes in salinity of rearing water. J. Korean Fish. Soc., 32, 310-316. (in Korean)
  7. Boeuf, G and P. Patrick, 2001. How should salinity influence iish growh. Com. Biochem. Physiol. Part C, 130, 411-423
  8. Min, B. H., C. Y Choi and Y. J. Chang, 2005. Comparison of physiological conditions on black porgy, Acanthopagrus schlegeli acclimated and reared in freshwater and seawater. J. Aquaculture, 18, 37-44. (in Korean)
  9. Tsuzuki, M. Y., K. Ogawa, C. A. Strussmann, M. Maita and F. Takashima, 2001. Physiological rεsponses during stress and subsequent recovery at different salinities in adult pejerrey Odontesthes bonariensis. Aquaculture, 200, 349-362 https://doi.org/10.1016/S0044-8486(00)00573-1
  10. Min, B. H., 2003. Physiological responses of black seabream, Acanthopagrus schlegeli to rreshwater acclimation. Master thesis, Pukylmg National University, Busan, Korea, 55 pp. (in korean)
  11. Pickford, G. E., P. K. T. Pang, E. weinstein, J. Torreli, E. Hendlcr and F.H. Epstein, 1970. The response of hypophysectomized cyprinodont, Fundulus heteroclitus, to replacemεnt therapy with cortisol: effects on blood serum and sodium-potassium activated adenosine triphosphatase in the gills, kidney and intestinal mucosa. Gen. Comp. Endocrinol., 14, 524-534 https://doi.org/10.1016/0016-6480(70)90036-5
  12. Carmichael,G. J., J. R. Tomasso, B.A. Simco and K.B Davis. 1984. Characterization and alleviation ofstress associated with hauling largemouth bass. Trans. Am. Fish. Soc., 113, 778-785 https://doi.org/10.1577/1548-8659(1984)113<778:CAAOSA>2.0.CO;2
  13. Specker, C. B., C. S. Bradford, M. S. Fitzpatrick and R. Patino, 1989. Regulation of the interrenal offishes: Non-classical control mechrulism. Fish Physiol. Biochem., 7, 259-265 https://doi.org/10.1007/BF00004715
  14. Wedemeyer, G. A. and D. J. McLeay, 1981. Methods for determining the tolerance of fishes to environmental stressors. In Stress and Fish (Ed. by A. D. Pickering), Academic Press, London, 247-275
  15. Barton, B. A. and G K. Iwama, 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and e:ffects of corticosteroids. Annu. Rev. Fish Dis., 1, 3-26 https://doi.org/10.1016/0959-8030(91)90019-G
  16. Park, M. R., Y. J. Chang and D. Y Kang, 1999. Physiological response of the cultured olive flo.undεr (Paralichthys olivaccus) to the acute changes of water temper따ure. J. Aquaculture, 12, 221-228. (in Korean)
  17. Chang, Y. J., B. H. Min, H. J. Chang and J. W. Hur, 2002. Comparison of blood physiology injuvenile black seabream (Acanthopagrus schlegeli) reared in converted freshwater from seawater and seawater from freshwater. J. Korean Fish. Soc., 35, 595-600. (ín Korean)
  18. Wedemeyer, G. A. and W. T Yasutake, 1977. Clinical methods for the assessment of the effects of environmental stress on fish health. U.S. Fish and Wildlife Service Technical Paper, 89, 18 pp. Washington D. C.
  19. Perry, S. F. and S. D. Reid, 1993. $\beta$-adrenergic signal transduction in fish : interactive e:ffects of catecholamines and cortisol. Fish. Physiol. Biochem., 11, 195-203 https://doi.org/10.1007/BF00004567
  20. Tomasso, J. R., K. B. Davis and N. C. Parker,1980. Plasma corticosteroid and electrolyte dynamics of hybrid striped bass (white bass x striped bass) during netting and hauling stress. Proc. World Maricult. Soc., 11, 303-310