Profiles of Plasma Sex Steroid Hormone and Vitellogenin According to Ovarian Development of the Oblong Rockfish Sebastes oblongus

황점볼락 난소 발달에 따른 혈중 성호르몬과 난황단백전구체의 변동

  • 김대현 (국립수산과학원 남해수산연구소) ;
  • 정지현 (한국해양연구원) ;
  • 윤성종 (국립수산과학원 양식관리과) ;
  • 황형규 (국립수산과학원 남해수산연구소) ;
  • 이윤호 (국립수산과학원 남해수산연구소) ;
  • 김대중 (국립수산과학원 남부내수면연구소)
  • Published : 2009.02.25

Abstract

To understand the steroidogenic activities and plasma vitellogenin (VTG) profiles according to the reproductive phases in the oblong rockfish Sebastes oblongus, we examined changes in sex steroid hormones and plasma vitellogenin. Plasma levels of testosterone (T) was significantly higher value in only ovulation stage (P<0.05). In vitellogenesis, plasma estradiol-$17{\beta}$ ($E_2$) had a high level in August which was a similar higher level until ovulation than other ovarian development stages (P<0.05). However, $E_2$ was significantly decreased after embryo stage (P<0.05). This indicates that variability in $E_2$ at different stage is associated with the development of the oocytes. Plasma levels of $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregnen-3-one (DHP) were significantly high at the stages of vitellogenesis and ovulation (P<0.001). It is assumed that DHP plays an important role in vitellogenesis. Also, We determined the plasma levels of vitellogenin (VTG) divided the development stage into four steps: immaturation, vitellogenesis, and ovulation and parturition. A significant lower levels of VTG were shown in immaturation and parturition (P<0.05), which did not discriminate between them. However, in vitellogenesis and ovulation were shown in a remarkable higher levels of VTG(P<0.05), but not significantly different between them. Consequently, plasma VTG levels were considerably increased after October and maintained a higher concentration until ovulation, but significantly decreased after ovulation. It is suggested that VTG plays also an important role in the development of vitellogenesis and oogenesis.

References

  1. Baek, J.M., C.H. Han, D.J. Kim, C.w. Park and K. Aida. 2000. Reproductive cycle of a rockfish, Sebaytes schlegeli. J. Kor. Fish. Soc., 33, 431-438
  2. Chang, Y. J., H. K. Lim, and J. Y. Kwon. 2001. Changes in plasma steroid hormone level in rockfish, Sebastes inermis by the controlled water temperature and phoperiod J Korean Fish. Soc., 34, 13-16
  3. Methven, D.A., L.W. Crim, B. Norberg, J.A. Brown, G.P. Goff and I. Huse. 1992. Seasonal reproduction and plasma levels of sex steroids and vitellogenin in Atlantic Halibut (Hippoglossus hippoglossus). Can. J. Fish. Aquat. Sci., 49, 754-759 https://doi.org/10.1139/f92-084
  4. Nagahama,Y., A. Takemura, K. Takano, S. Adachi and M. Kusakari. 1991. Serum steroid hormone levels in relation to the reproductive cycle of Sebastes taczanowskii and S. schlegeli. Environ. Biol. Fishes, 30, 31-38 https://doi.org/10.1007/BF02296874
  5. Nagaharna, Y. 1997. 17a, 20${\ss}$-dihydroxy-4-pregnen-3-one, a maturation- inducing hormone in fish oocyte: Mechanisms of synthesis and action. Steroids, 62, 190-196 https://doi.org/10.1016/S0039-128X(96)00180-8
  6. Park, M.H., I.J. Hwang, D.J. Kim, Y.D. Lee, H.B. Kim and H.J Baek. 2005. Gonadal Development and sex steroid hormone levels ofthe yellowfin goby Acanthogobiusflavimanus. J. Kor. Fish. Soc., 38, 309-315
  7. Sun, B. and N.W. Pankhurst. 2004. Patterns of oocyte growth, vitellogenin and gonadal steroid concentrations in greenback f1onnder. J. Fish Biol., 64, 1399-1412 https://doi.org/10.1111/j.0022-1112.2004.00406.x
  8. Aida,K. 1988. A review of plasma hormone changes during ovulation in cyprinid fishes. Aquaculture, 74, 11-21 https://doi.org/10.1016/0044-8486(88)90081-6
  9. Aida, K. 1991. Environmental regulation of reproductive rhythums on teleosts. Bull. Inst. Zool., Academia Sinica, Monograph, 16, 173-187
  10. Kwak, E. J., K. W. Lee, S. W. Yang, N. H. Choi, C. K. Park, K. H. Han, and W. K. Lec. 2006. Gonadal dcvelopment and sex ratio of artificial seedlings of the oblong rockfish Sebastes oblongus. J. Kor. Fish. Soc., 39, 297-302
  11. Rahman,M.S., A. Takemura and K. Takano. 2000. Annual changes in ovarian histology, plasma steroid hormones and vitellogenin in tbe female golden rabbitfish, Sigamus guttatus (Bloch). Bull. Mar. Sci., 67, 729-740
  12. Kokokiris, L., B. Mourot, F. Le Menn, M. Kentouri and A. Fostier. 2000. Endocrine changes during the annual reproductive cycle of the red porgy, Pagrus pagrus (Teleostei, Sparidae). Fish Physiol. Biochem., 23, 1-11 https://doi.org/10.1023/A:1007882807782
  13. Ko, C. S., Y. J. Chang, H. K. Lim, J. H. Kim, and K. C. Cho. 1998. Controlled reproductive cycle ofrockfish (Sebastes inermis) by water temperature and photoperiod. J. Korean Fish. Soc. 31, 713-720
  14. Kim, D. J., C. M. An and K. S. Min. 2006. Plasma sex steroid hormone and vitellogenin profiles during ovarian development of the wild marbled sole, Limaηda yokohamαe. J. Kor. Fish. Soc. 39, 391-397
  15. Matsuyarna, M., S. Adachi, Y. Nagaharna, C. Kitajima and S. Matsuura. 1991. AnnuaJ reproductive cycle of the captive femaJe Japanese sardine Sardinops melanostictus: relationship to ovarian development and serum levels of gonadal steroid hormones. Mar. Biol., 108, 21-29 https://doi.org/10.1007/BF01313467
  16. Yoon, S. J., D. H. Kim, H. G. Hwang, G. C. Song, and Y. C. Kim. 2007. Effects ofwater temperature, stocking density and feeding frequency on survival and growth in the oblong rockfish Sebastes oblongus larvae. Korean J. Ichthyol., 19, 154-159
  17. Kim, D. J., J. Y. Bae and E. O. Kim. 2007. Changes in sex steroid hormones and ovarian development during artificial maturation of female eel, A nguilla japonica. Integrative Biosciences, 11, 117-124 https://doi.org/10.1080/17386357.2007.9647323
  18. Mandich, A., A. Massari, S. Bottero, P. Pizzicori, H. Goos and G. Marino. 2004. Plasma sex steroid and vitellogenin profiles during gonadal development in wild Mediterranean arnberjack (Seriola dumerilii). Mar. Biol., 144, 127-138 https://doi.org/10.1007/s00227-003-1185-6
  19. Koya, Y., K. Soyano, K. Yarnarnoto, H. Obana and T. Matsubara. 2003.Oocyte development and serum profiles of vitellogenin and steroid hormone levels in captive female Pacific heηing Clupea pallasii during their first maturational cycle. Fisher. Sci., 69, 137-145 https://doi.org/10.1046/j.1444-2906.2003.00598.x
  20. 勝田失郞. 1958. 服牛魚タヶノコメパルの卵發生と子魚期. 日水誌.. 24, 475-479
  21. Chang, Y. J., H. K. LIm, and S. G. Byun. 1995. Gonadal maturation and reproductive cycle in oblong rockfish, Sebastes oblongus. J Aquacult., 8, 31-46
  22. Tsuruda, Y. and K. Hirose. 1989. Internal regulation of reproduction in Japanese anchovy (Engraulis japonica) as related to population fluctuation. Can. Spec. Pub. Fish. Aquatic Sci., 108, 111-119
  23. Jung, H. J., D. J. Kim and C. H. Han. 2004. Rockfish (Sebastes schlegeli) vitelIogenin: Purification, characterization and development of sandwich ELISA system. J. Fish. Sci. Tech., 7, 99-108 https://doi.org/10.5657/fas.2004.7.3.099