Extracts from Rhizopus oryzae KSD-815 of Korean Traditional Nuruk Confer the Potential to Inhibit Hypertension, Platelet Aggregation, and Cancer Metastasis in vitro

  • Lee, Sang-Jin (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Bae, Hyun-Jin (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Ryu, Ji-Yeon (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Lee, Dae-Young (Natural Products Chemistry Laboratory, Department of Biotechnology, Kyung Hee University) ;
  • Kim, Gye-Won (Research Laboratories, Kooksoondang Brewery Co., Ltd.) ;
  • Baek, Na-Min (Natural Products Chemistry Laboratory, Department of Biotechnology, Kyung Hee University) ;
  • Kwon, Moo-Sik (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Hong, Sung-Youl (Department of Genetic Engineering, Sungkyunkwan University)
  • Published : 2009.12.31


Rhizopus oryzae KSD-815 was isolated from nuruk that has been used to make Korean traditional wines. This study was performed to investigate the effect of cultures of R. oryzae KSD-815 on cardiovascular disorders and cancer metastasis. Firstly, these cultures were sequentially fractionationed with n-hexane (TAHe), ethylacetate (TAE), n-butanol (TAB), and $H_2O$ (TAW). The TAE inhibited the activity of angiotensin-converting enzyme (ACE) and TAB suppressed platelet aggregation in vitro. TAE and TAB inhibited cell motility of human breast cancer cells. Furthermore, TAW interrupted the formation of neovasculature and tube-like structure, and down-regulated the expression of angiogenic factors, basic fibroblast growth factor (bFGF), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), and hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) in breast cancer cells. These results indicated that cultures of R. oryzae KSD-815 display the inhibitory activities on hypertension, platelet aggregation, and metastasis, and suggest that these cultures might be further probed for the purposes as therapeutic agents or dietary supplements.


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