Purification, Chemical Composition, and in vitro Antioxidant Activity of Two Protein-bound Polysaccharides from Rapeseed Meal

  • Sun, Han-Ju (School of Biotechnology and Food Engineering, Hefei University of Technology) ;
  • Jiang, Shaotong (School of Biotechnology and Food Engineering, Hefei University of Technology) ;
  • Zi, Mingyang (Department of Food Science and Technology, South China University of Technology) ;
  • Qi, Ding (School of Biotechnology and Food Engineering, Hefei University of Technology)
  • Published : 2009.12.31


Crude polysaccharides from rapeseed meal (PRM) were extracted with 0.3% NaOH aqueous solution, followed by further purifications and 2 fractions, namely PRM1 and PRM2, were separated with a DEAE-cellulose DE-52 column. Their primary compositions were analysed and antioxidant activity was determined, including scavenging activity toward superoxide anion radicals, hydroxyl radicals, and nitric oxide radicals, reducing power, and inhibitory effects against the microsomal lipid peroxidation, compared to that of L-ascorbic acid. The results indicated that PRM1 and PRM2 exhibited not only good reducing power and inhibitory effects on the microsomal lipid peroxidation, but also strong scavenging activity toward superoxide anion radicals, nitric oxide radicals, and hydroxyl radicals. In addition, positive correlations were also observed between the superoxide anion radical scavenging activity and the protein contents of the polysaccharides, and the reducing power and the sulfate contents. These findings thus clearly suggest the polysaccharides possess direct and potent antioxidant activity.


  1. Xiang ZN, Ning ZX. Scavenging and antioxidant properties of compound derived from chlorogenic acid in South-China honeysuckle. LWT-Food Sci. Technol. 41: 1189-1203 (2008) https://doi.org/10.1016/j.lwt.2007.08.006
  2. Rathee JS, Hassarajani SA, Chattopadhyay S. Antioxidant activity of Mammea longifolia bud extracts. Food Chem. 99: 436-443 (2006) https://doi.org/10.1016/j.foodchem.2005.08.020
  3. Siddhuraju P, Manian S. The antioxidant activity and free adicalscavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem. 105: 950-958 (2007) https://doi.org/10.1016/j.foodchem.2007.04.040
  4. Schepetkin IA, Quinn MT. Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential. Int. Immunol. 6: 317-333 (2006) https://doi.org/10.1016/j.intimp.2005.10.005
  5. Baethgen WE, Alley MM. A manual colorimetric procedure for measuring ammonium nitrogen in soil and plant Kjeldahl digests. Commun. Soil Sci. Plan. 20: 961-969 (1989) https://doi.org/10.1080/00103628909368129
  6. Dong CH, Yao YJ. In vitro evaluation of antioxidant activities of aqueous extracts from natural and cultured mycelia of Cordyceps sinensis. LWT-Food Sci. Technol. 41: 669-677 (2008) https://doi.org/10.1016/j.lwt.2007.05.002
  7. Rajapakse N, Mendis E, Jung WK, Je JY, Kim SK. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Res. int. 38: 175-182 (2005) https://doi.org/10.1016/j.foodres.2004.10.002
  8. Kawai Y, Seno N, Anno K. A modified method for chondrosulfatase assay. Anal. Biochem. 32: 314-321 (1969) https://doi.org/10.1016/0003-2697(69)90091-8
  9. Bruckdorfer R. The basics about nitric oxide. Mol. Aspects Med. 26: 3-31 (2005) https://doi.org/10.1016/j.mam.2004.09.002
  10. Yang B, Jiang Y, Zhao M, Shi J, Wang L. Effects of ultrasonic extraction on the physical and chemical properties of polysaccharides from longan fruit pericarp. Polym. Degrad. Stabil. 93: 268-272 (2008) https://doi.org/10.1016/j.polymdegradstab.2007.09.007
  11. Huang X, Wang D, Hu Y, Lu Y, Guo Z, Kong X, Sun J. Effect of sulfated astragalus polysaccharide on cellular infectivity of infectious bursal disease virus. Int. J. Biol. Macromol. 42: 166-171 (2008) https://doi.org/10.1016/j.ijbiomac.2007.10.019
  12. Magalhaes LM, Segundo MA, Reis S, Lima JLFC. Methodological aspects about in vitro evaluation of antioxidant properties. Ana. Chim. Acta 613: 1-19 (2008) https://doi.org/10.1016/j.aca.2008.02.047
  13. Wang J, Zhang Q, Zhang Z, Li Z. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 42: 127-132 (2008) https://doi.org/10.1016/j.ijbiomac.2007.10.003
  14. Qi H, Zhang Q, Zhao T, Chen R, Zhang H, Niu X, Li Z. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int. J. Biol. Macromol. 37: 195-199 (2005) https://doi.org/10.1016/j.ijbiomac.2005.10.008
  15. Bitter T, Muir HM. A modified uronic acid carbazole reaction. Anal. Biochem. 330-334 (1962)
  16. Cacciuttolo MA, Speca D, Renard P, Lumpkin J, Remacle J, Rao G. DNA damage by hyperoxia in hybridoma cells is mediated by metal ions and hydroxyl radicals. Free Radical Bio. Med. 15: 486 (1993)
  17. Gulcin I. Antioxidant and antiradical activities of L-carnitine. Life Sci. 78: 803-811 (2006) https://doi.org/10.1016/j.lfs.2005.05.103
  18. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 (1956) https://doi.org/10.1021/ac60111a017
  19. Wu WM, Lu L, Long Y, Wang T, Liu L, Chen Q, Wang R. Free radical scavenging and antioxidative activities of caffeic acid phenethyl ester (CAPE) and its related compounds in solution and membranes: A structure-activity insight. Food Chem. 105: 107-115 (2007) https://doi.org/10.1016/j.foodchem.2007.03.049
  20. Li XL, Zhou AG, Han Y. Anti-oxidation and anti-microorganism activities of purification polysaccharide from Lygodium japonicum in vitro. Carbohyd. Polym. 66: 34-42 (2006) https://doi.org/10.1016/j.carbpol.2006.02.018
  21. Sun H, Jiang S, Mu P, Qi D. In vivo antioxidative capacities of rapeseed meal polysaccharides. J. Food Agric. Environ. 7: 97-102 (2009)
  22. Halliwell B. Phagocyte-derived reactive species: Salvation or suicide? Trends Biochem. Sci. 31: 509-515 (2006) https://doi.org/10.1016/j.tibs.2006.07.005
  23. Qian ZJ, Jung WK, Byun HG, Kim SK. Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Bioresource Technol. 99: 3365-3371 (2008) https://doi.org/10.1016/j.biortech.2007.08.018
  24. Bae IY, Oh IO, Lee S, Yoo S, Lee HG. Rheological characterization of levan polysaccharides from micro bacterium laevaniformans. Int. J. Biol. Macromol. 42: 10-13 (2008) https://doi.org/10.1016/j.ijbiomac.2007.08.006
  25. Alonso-Sande M, Teijeiro-Osorio D, Remunan-Lopez C, Alons MJ. Glucomannan, a promising polysaccharide for biopharmaceutical purposes. Eur. J. Pharm. Biopharm. 2: 1-10 (2008)
  26. Awah FM, Uzoegwu PN, Oyugi JO, Rutherford J, Ifeonu P, Yao XJ, Fowke KR, Eze MO. Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract. Food Chem. 9: 1-8 (2009) https://doi.org/10.1016/0308-8146(82)90064-4
  27. Srivastava A, Harish SR, Shivanandappa T. Antioxidant activity of the roots of Decalepis hamiltonii (Wight & Arn.). LWT-Food Sci. Technol. 39: 1059-1065 (2006) https://doi.org/10.1016/j.lwt.2005.07.005