DOI QR코드

DOI QR Code

Fatty Acid Composition in Blood Plasma and Follicular Liquid in Cows Supplemented with Linseed or Canola Grains

  • Perehouskei Albuquerque, Karina ;
  • do Prado, Ivanor Nunes ;
  • Bim Cavalieri, Fabio Luiz ;
  • Rigolon, Luiz Paulo ;
  • do Prado, Rodolpho Martin ;
  • Pizzi Rotta, Polyana
  • Received : 2008.10.04
  • Accepted : 2009.03.15
  • Published : 2009.09.01

Abstract

This study was carried out to evaluate the fatty acid composition in Nellore cows supplemented with either linseed (n-3) or canola grains (n-6 and n-9). Fifteen Nellore cows, aged five years and bodyweight 550 kg${\pm}$48 kg, were randomly distributed to the following treatments: CON (control), LIN (linseed) and CAN (canola grains). The cows were fed for 80 days. The concentrations of C18:0, C18:2 n-6 and C20:3 n-6 fatty acid were higher (p<0.10) in CON blood plasma in comparison to follicular liquid. Likewise, PUFA, n-6 contents, PUFA:SFA and n-6:n-3 ratios were higher (p<0.10) in blood plasma. On the other hand, C18:1 n-9, C22:5 n-3, MUFA and n-3 contents were lower (p<0.10) in blood plasma. C18:0, C18:2 n-6, C18:3 n-3, C22:5 n-3, PUFA, n-6, n-3 contents and PUFA:SFA ratio were higher (p<0.10) in LIN blood plasma than in the follicular liquid. Nevertheless, C14:0, C16:0, C16:1 n-7, PUFA, C16:0, C18:1 n-9 and MUFA contents were lower (p<0.10) in LIN blood plasma. On treatment CAN, the C18:0 and SFA contents, and n-6:n-3 ratios were higher (p<0.10) in blood plasma. However, C20:3 n-6, C22:5 n-3, PUFA and n-3 contents were lower (p<0.10) in blood plasma. C16:0, C18:0, PUFA, SFA contents and PUFA:SFA ratio did not differ (p>0.10) among the treatments. C14:0, C16:1 n-7, C18:2 n-6 and n-6 contents were higher (p<0.10) for CON and CAN than LIN. C17:1 n-7, C20:4 n-6 and C 22:0 contents were higher (p<0.10) for CAN than CON and LIN. C18:1 n-9, C18:3 n-3, MUFA and n-3 contents were higher (p<0.10) for LIN and CAN than CON. C20:3 n-6 content and n-6:n-3 ratio were higher (p<0.10) for CON than LIN and CAN. C22:5 n-3 content were higher (p<0.10) for CON and LIN than CAN. The concentrations of fatty acids in blood plasma and follicular liquid were not correlated for any fatty acid, independent of the treatment studied. Canola grain added to the diet of Nellore cows resulted in increased concentrations of fatty acids n-6 and n-3 in follicular liquid.

Keywords

Biohydrogenation;Fatty Acids;Nellore;Omega-3;Omega-6

References

  1. Bauchart, D. 1993. Lipid absorption and transport in ruminants. J. Dairy Sci. 76:3864-3881 https://doi.org/10.3168/jds.S0022-0302(93)77728-0
  2. Folch, J., M. Less and M. Sloane-Stanley. 1957. Isolation and purification of total lipids from tissues. J. Biol. Chem. 226:497-509
  3. McEvoy, T. G., G. D. Coull, P. J. Broadbent, J. S. M. Hutchinson and B. K. Speake. 2000. Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. Reprod. 118:163-170 https://doi.org/10.1530/reprod/118.1.163
  4. Khandoker, M. A. M., H. Tsujii and D. Karasawa. 1997. Fatty acid composition of oocytes, follicular, oviductal and uterine fluids of pig and cow. Asian-Aust. J. Anim. Sci. 10:523-527
  5. Guillevic, M., M. Kouba and J. Mourot. 2009. Effect of linseed diet on lipid composition, lipid peroxidation and consumer evaluation of French fresh and coocked pork meats. Meat Sci. 81:612-618 https://doi.org/10.1016/j.meatsci.2008.10.019
  6. Zeron, Y., M. Tomczak, J. Crowe and A. Arav. 2002. The effect of liposomes on thermotropic membrane phase transitions of bovine spermatozoa and oocytes: implications for reducing chilling sensivity. J. Reprod. Fert. 45:143-152 https://doi.org/10.1016/S0011-2240(02)00123-2
  7. Oliveira, S. G., J. M. C. Simas and F. A. P. Santos. 2004. Main aspects related to changes in the profile of fatty acids in ruminant milk fat. Arch. Veter. Sci. 9:73-80
  8. Petit, H. V. 2003. Digestion, milk production, milk composition and blood composition of dairy cows fed formaldehyde treated flaxseed or sunflower seed. J. Dairy Sci. 86:2637-2646 https://doi.org/10.3168/jds.S0022-0302(03)73859-4
  9. Tamminga, S. and M. Doreau. 1991. Lipids and rumen digestion. In: Rumen microbial metabolism and ruminant digestion (Ed. J. P. Jouany). INRA Editions, Paris. pp. 151-163
  10. Harfoot, C. G. and G. P. Hazlewood. 1997. Lipid metabolism in the rumen. In: The rumen microbial ecosystem (Ed. P. N. Hobson and C. S. Stwart). Blackie Academic. pp. 382-426
  11. Souza, N. E. and J. V. Visentainer. 2006. Acidos graxos. In: Colesterol da mesa ao corpo (Ed. N. E. Souza and J. V. Visentainer). 1.ed. Sao Paulo: Varela. pp. 14-42
  12. National Research Council (NRC). 1996. Nutrient requirements of beef cattle. $7^{th}$ Revised Edition, National Academy Press. Washington, DC. p. 242
  13. Petit, H. V. 2002. Digestion, milk production, milk composition, and blood composition of dairy cows fed whole flaxseed. J. Dairy Sci. 85:1482-1490 https://doi.org/10.3168/jds.S0022-0302(02)74217-3
  14. Reichenbach, H. D., M. A. L. Oliveira and P. F. Lima. 2002. Transferencia e criopreservacao de embriao bovino. Biotecnicas aplicada a reproducao bovina. 1.ed. Sao Paulo: Varela. p. 560
  15. Stubbs, C. D. and A. D. Smith. 1984. The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Bioch. Bioph. Acta. 779:89-137 https://doi.org/10.1016/0304-4157(84)90005-4
  16. Palmquist, D. L. and T. C. Jenkis. 1980. Fat in lactation rations. J. Dairy Sci. 63:1-14 https://doi.org/10.3168/jds.S0022-0302(80)82881-5
  17. Yao, J. K., R. J. Ryan and P. J. Dyck. 1980. The porcine ovarian follicle. VI Comparison of fatty acid composition of serum and follicular fluid at different development stages. Biol. Reprod. 22:141-147 https://doi.org/10.1095/biolreprod22.2.141
  18. Coppock, C. E. and D. L. Wilks. 1991. Supplemental fat in highenergy rations for lactating cows: effects on intake, digestion, milk yield, and composition. J. Anim. Sci. 69:3826-3837
  19. Prado, I. N., R. H. Ito, J. M. Prado, I. M. Prado, P. P. Rotta, M. Matsushita, J. V. Visentainer and R. R. Silva. 2008. The influence of dietary soyabean and linseed on the chemical composition and fatty acid profile of the Longissimus muscle of feedlot-finished bulls. J. Anim. Feed Sci. 17:307-317
  20. Cavalieri, F. L. B., G. T. Santos, M. Matsushita, H. Petit, L. P. Rigolon, D. C. Silva, J. A. Horst, L. C. T. Capovilla and F. S. Ramos. 2005. Milk production and milk composition of dairy cows fed Lac100 or flaxseed. Can. J. Anim. Sci. 85:413-416 https://doi.org/10.4141/A04-088
  21. Zeron, Y, ., A. Ocheretny, O. Kedar, A. and A. ABorochovD. Sklan rav. 2001. Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. J. Reprod. Fertz121:447-454 https://doi.org/10.1530/rep.0.1210447