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The bio-complex "reaction pattern in vertebrate cells" reduces cytokine-induced cellular adhesion molecule mRNA expression in human endothelial cells by attenuation of NF-kappaB translocation

  • Ronnau, Cindy (Department of Internal Medicine B, University of Greifswald) ;
  • Liebermann, Herbert E. H. (Varicula Life Science AG) ;
  • Helbig, Franz (Varicula Life Science AG) ;
  • Staudt, Alexander (Department of Internal Medicine B, University of Greifswald) ;
  • Felix, Stephan B. (Department of Internal Medicine B, University of Greifswald) ;
  • Ewert, Ralf (Department of Internal Medicine B, University of Greifswald) ;
  • Landsberger, Martin (Department of Internal Medicine B, University of Greifswald)
  • Published : 2009.02.28

Abstract

The bio-complex "reaction pattern in vertebrate cells"(RiV) is mainly represented by characteristic exosome-like particles - probably as reaction products of cells to specific stress. The transcription factor NF-kappaB plays a central role in inflammation. We tested the hypothesis that RiV particle preparations (RiV-PP) reduce cellular adhesion molecule (CAM) expression (ICAM-1, VCAM-1, E-selectin) by the attenuation of NF-kappaB translocation in human umbilical vein endothelial cells (HUVEC). After 4 hours, pre-incubation of HUVEC with RiV-PP before stimulation with TNF-alpha significantly reduced ICAM-1 (65.5${\pm}$10.3%) and VCAM-1 (71.1${\pm}$12.3%) mRNA expression compared to TNF-alpha-treated cells (100%, n=7). ICAM-1 surface expression was significantly albeit marginally reduced in RiV/TNF-alpha- treated cells (92.0${\pm}$5.6%, n=4). No significant effect was observed on VCAM-1 surface expression. In RiV/TNF-alpha-treated cells (n=4), NF-kappaB subunits p50 (85.7${\pm}$4.1%) and p65 (85.0${\pm}$1.8%) nuclear translocation was significantly reduced. RiV-PP may exert an anti-inflammatory effect in HUVEC by reducing CAM mRNA expression via attenuation of p50 and p65 translocation.

Keywords

Endothelial function;E-selectin;ICAM-1;Inflammation;VCAM-1

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