Analysis of residual neomycin in honey by LC-MS/MS

LC-MS/MS에 의한 벌꿀 중 잔류 네오마이신의 분석

  • 심영은 (경기대학교 자연과학대학 화학과) ;
  • 정지윤 (식품의약품안전청 식품잔류약품과) ;
  • 명승운 (경기대학교 자연과학대학 화학과)
  • Received : 2009.02.02
  • Accepted : 2009.07.03
  • Published : 2009.08.25

Abstract

An effective and specific procedure for confirmation of neomycin, aminoglycoside antibiotic in honey was developed and validated. Honey was adjusted to pH 2 with 0.1M HCl and applied to weak cation-exchange SPE cartridge. Neomycin was eluted with basified methanol. Following separation by ion-pairing liquid chromatography, neomycin was analysed with positive electrospray ionization and MRM mode. Quantification was linear over the range of $5.0{\sim}250.0{\mu}g/kg$ ($r^2$ >0.9951). The precision (R.S.D.) and accuracy (as a bias) of quality control samples in honey ranged 11.5~18.7% and 10.9~20.9%, respectively. Established method can be applied to analysis of neomycin in honey.

Keywords

Honey;neomycin;LC-MS/MS

Acknowledgement

Grant : 벌꿀 중 항생제 분석법 개발 및 모니터링

Supported by : 식품의약품안전청

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