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cDNA Microarray Analysis of Differential Gene Expression in Boar Testes during the Prepubertal Period

  • Lee, Dong-Mok (School of Biotechnology, Yeungnam University) ;
  • Lee, Ki-Ho (Department of Biochemistry and Molecular Biology, and Medical Sciences Research Institute, Eulji University) ;
  • Choi, Jin Ho (School of Biotechnology, Yeungnam University) ;
  • Hyun, Jin Hee (Department of Biotechnology, Yeungnam University) ;
  • Lee, Eun Ju (Department of Biotechnology, Yeungnam University) ;
  • Bajracharya, Prati (Department of Biotechnology, Yeungnam University) ;
  • Lee, Yong Seok (Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Chang, Jongsoo (Department of Agricultural Sciences, Korea National Open University) ;
  • Chung, Chung Soo (Department of Animal Science, Chungbuk National University) ;
  • Choi, Inho (School of Biotechnology, Yeungnam University,Department of Biotechnology, Yeungnam University)
  • Received : 2009.01.02
  • Accepted : 2009.04.02
  • Published : 2009.08.01

Abstract

In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized complementary DNA (cDNA) from boar testes was generated. DNA sequencing of 2,016 randomly selected clones yielded 794,116 base pairs of high quality nucleotide sequence. Computer-assisted assembly of the nucleotide sequence of each clone resulted in 423 contigs and 403 singletons including several genes for steroidogenic enzymes and molecules related to steroid metabolism. Analysis of gene expression pattern by use of the presently-fabricated cDNA microarray identified a number of genes that were differentially expressed during the postnatal development period in boar testes. Two genes of unknown function were identified to be highly expressed in the testis of 2-weeks-old neonatal boar. In addition, the sequencing of open reading frames of these genes revealed their homology with human alpha hemoglobin and Homo sapiens hypothetical LOC643669, transcript variant 1. Moreover, the transcripts of these genes were also detected in porcine muscle and adipocytes, in addition to Leydig cells of pigs.

Acknowledgement

Supported by : Rural Development Administration

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