Enzymatic properties of the N- and C-terminal halves of human hexokinase II

  • Ahn, Keun-Jae ;
  • Kim, Jong-Sun ;
  • Yun, Mi-Jin ;
  • Park, Jeon-Han ;
  • Lee, Jong-Doo
  • Published : 2009.06.30


Although previous studies on hexokinase (HK) II indicate both the N- and C-terminal halves are catalytically active, we show in this study the N-terminal half is significantly more catalytic than the C-terminal half in addition to having a significantly higher $K_m$ for ATP and Glu. Furthermore, truncated forms of intact HK II lacking its first N-terminal 18 amino acids ($\Delta$18) and a truncated N-terminal half lacking its first 18 amino acids ($\Delta$18N) have higher catalytic activity than other mutants tested. Similar results were obtained by PET-scan analysis using $^{18}F-FDG$. Our results collectively suggest that each domain of HK II possesses enzyme activity, unlike HK I, with the N-terminal half showing higher enzyme activity than the C-terminal half.


Conformation;Deletion mutant;Hexokinase II;Kinetics properties;$^{18}F-FDG$


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