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Rice OsACDR1 (Oryza sativa Accelerated Cell Death and Resistance 1) Is a Potential Positive Regulator of Fungal Disease Resistance

  • Kim, Jung-A (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Cho, Kyoungwon (Environmental Biology Division, National Institute for Environmental Studies (NIES)) ;
  • Singh, Raksha (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Jung, Young-Ho (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Jeong, Seung-Hee (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Kim, So-Hee (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Lee, Jae-eun (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Cho, Yoon-Seong (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Agrawal, Ganesh K. (Research Laboratory for Biotechnology and Biochemistry) ;
  • Rakwal, Randeep (Research Laboratory for Biotechnology and Biochemistry) ;
  • Tamogami, Shigeru (Laboratory of Biologically Active Compounds, Department of Biological Production, Akita Prefectural University) ;
  • Kersten, Birgit (Max Planck Institute for Molecular Plant Physiology, GabiPD Team, Bioinformatics) ;
  • Jeon, Jong-Seong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • An, Gynheung (National Research Laboratory, Department of Life Science and Center for Functional Genomics, Pohang University of Science and Technology) ;
  • Jwa, Nam-Soo (Department of Molecular Biology, College of Natural Science, Sejong University)
  • Received : 2009.07.03
  • Accepted : 2009.10.08
  • Published : 2009.11.30

Abstract

Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.

Acknowledgement

Supported by : Korea Science and Engineering Foundation, Ministry of Science and Technology, Ministry of the Environment

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